炎症微环境对于牙周膜干细胞与脐带间充质干细胞再生功能的影响

doi:10.3969/j.issn.1674-8603.2020.02.002

Abstract

Abstract: Objective:The study aims to compare effect of inflammatory microenvironment on the regenerative capacitary of periodontal ligament stem cells (PDLSCs) andumbilical cord mesenchymal stem cells (UCMSCs). Methods：TNF-α was applied to mimic inflammatory microenvironment. Cell proliferation assay and proliferation marker genes CCNE1, CCND1 analysis were used to compare the proliferation capacity. AnnexinV/PI staining was used to detect cellular apoptosis. Alizarin red staining and osteogenesis marker genes RUNX2, OCN analysis were used to compare the capacity of osteogenic differentiation. Results：Treatment of TNF-α resulted in an obvious decrease in the proliferation and osteogenic differentiation capacity of PDLSCs, while an increase in apoptosis of PDLSCs. In contract, treatment of TNF-α leaded to a mild decrease in the capacity of proliferation and osteogenic differentiation in UCMSCs, and a slight increase in cellular apoptosis. Conclusions：Comparing with PDLSCs, UCMSCs are more resistant to the influence of inflammatory microenvironment.

Key words: periodontal ligament stem cells, Human umbilical cord mesenchymal stem cells, Inflammation, TNF-α, differentiation, proliferation

References 13

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The Influence of inflammatory micro-environment on regenerative capacity of PDLSCs and UCMSCs

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