持续激活Fgf8对颌面部不同来源成肌细胞的差异性作用

Abstract

Abstract: Objective：The number and proliferation level of myoblasts derived from the unsegmented paraxial mesoderm are lower than those of the somite-derived myoblasts after muscle injury. This study aims to investigate the effect of Fgf8 activation on the tongue myoblasts (derived from the somite) and the masseter myoblasts (derived from the unsegmented paraxial mesoderm) in the craniofacial region. Methods：A mouse model (Myf5-cre; Rosa26R-Fgf8, RF8) that specifically activates Fgf8 in myogenic progenitor cells and a red-green dual-fluorescence mouse model called Myf5-cre;mT/mG were used to obtain mouse embryos of different days that were soon processed. Masson staining was performed to observe the morphology of tongue and masseter muscle. Immunohistochemistry was used to detect the expression of Myosin and MyoD in the muscle. BrdU labeling assay was used to assess the cell proliferation. Results：Masson staining showed that Myf5-cre; Rosa26R-Fgf8 mice had disordered tongue muscle fibers, but no significant changes in masseter muscle. Immunohistochemical results showed that the reduction of Myosin in tongue muscle was significantly greater than that of masseter muscle. The increase of MyoD in the genioglossus muscle was significantly greater than that of the masseter muscle, but the expression of MyoD in the intrinsic tongue muscle decreased dramaticly. BrdU labeling assay showed that the growth rate of the proliferation of genioglossus myoblasts was significantly greater than that of masseter muscle, but there was no significant change in the intrinsic tongue muscle. Conclusions：Fgf8 activation significantly promotes the proliferation of genioglossus myoblasts derived from the somite and masseter myoblasts derived from the unsegmented paraxial mesoderm and inhibits their differentiation. Its regulating effect on the genioglossus muscle is significantly greater than that of the masseter muscle. In addition, Fgf8 activation did not promote the proliferation of somite-derived intrinsic tongue myoblasts, but its inhibitory effect on the differentiation was also greater than that of masseter myoblasts

Key words: Fgf8, tongue, masseter, myogenic differentiation

References 29

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Differential effects of Fgf8 on the myoblasts derived from different sources in the craniofacial region

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