慢性睡眠剥夺对大鼠颞下颌关节的影响

Abstract

Abstract: Objective:?To study the effects of chronic sleep deprivation on the histology of temporomandibular joint (TMJ) in rats. Methods:?60 rats were randomly divided into control group and experimental group. The rats were then chronicly deprived of sleep for 4, 6 and 8 weeks by the modified multiple platform method (MMPM). Finally, the pathological changes, angiogenesis, and the expression of vascular endothelial growth factor (VEGF) of TMJ condylar cartilage were evaluated by hematoxylin and eosin (HE) staining, CD105 fluorescent staining, and immunohistochemistry, respectively. Results:?MMPM can induce the rats into chronic sleep deprivation state successfully. HE staining showed osteoarthritic (OA)-like pathological changes, such as fiber fracture, reduced chondrocytes, disordered arrangement, and increased capillaries in the experimental group, while no changes were observed in the control group. The osteochondral junction MVDs of the experimental group were significantly higher than that of the control group (P <0.05)，and showed increasing trend with the prolonged sleep deprivation. Immunohistochemisty showed that VEGF was highly expressed in the middle and even superficial layers of the condyle cartilage in the experimental group, while the expression was relatively lower in each layer of the condyle cartilage in the control group. Conclusions:?Sleep deprivation of rats through MMPM could induce angiogenesis of TMJ condylar cartilage and TMJ-OA like histological changes in rats, which showed progressive enhancement in a time-dependent manner.

Key words: Chronic sleep deprivation, temporomandibular joint, angiogenesis, VEGF

References 27

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Effects of Chronic Sleep Deprivation on Rat' Temporomandibular Joint

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