Oral Biomedicine ›› 2021, Vol. 12 ›› Issue (1): 17-20.

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Guanine nucleotide exchange factor Trio effects craniofacial development by regulating neural crest cells migration

  

  • Received:2020-09-10 Revised:2020-12-25 Online:2021-03-25 Published:2021-04-01

Abstract: Objective:To investigate the effect of Rho GEF Trio on neural crest cells during embryonic development. Methods:Trio function was inhibited by microinjection of morpholino (MO) into zebrafish embryos,the development of mandibular and neural crest cells derivative was observed at 96 h through stereo microscope, and the development of craniomaxillofacial cartilage was observed at 120 h by Alcian blue staining. The effect of trio on the neural crest cells migration path of zebrafish was observed by using the neural crest cell specific labeling technique of transgenic zebrafish (sox10:EGFP)by means of Timelapse. Trio knockdown neural crest cells were cultured in vitro to observe the effect of Trio on the migration of neural crest cells through measuring the wound area (%) after migration in wound healing assay. Results:When trio function was inhibited,zebrafish mandibular development was insufficient, the Meckel's cartilage became retrusive, and the distance of neural crest cells migration decreased. In vitro,the wound area after neural crest cell migration increased in the Trio knockdown group. Conclusions:Trio may promote craniofacial development of neural crest origin by regulating the neural crest cells migration.

Key words:

Trio;?neural crest cells, ?migration;?craniofacial development;?zebrafish