基于RANKL/OPG通路探讨GsMTx4对大鼠正畸牙齿移动过程中压力侧牙周组织改建的影响

Abstract

Abstract: Objective: To explore the effect of the inhibitor GsMTx4 of Piezo1 protein channel on periodontal tissue reconstruction at the pressure side during orthodontic tooth movement in rats through RANKL/OPG pathway. Methods: The orthodontic model was established in 75 healthy male SD rats. The rats were randomly divided into control group, force group and force+GsMTx4 group. The mesial movement distance of the first molars was measured. HE staining was used to observe the pathological changes of periodontal tissue in the pressure side of the proximal root of the mandibular first molars. Immunohistochemical staining was used to observe the expression and localization of nuclear factor-κB receptor activator ligand (RANKL) and OPG in the periodontal tissue at pressure side. The change of the ratio of RANKL to osteoprotegerin (OPG) and the number of osteoclasts in periodontal tissues at the pressure side through tartrate-resistant acid phosphatase staining were observed. Results:With the extension of force time, the tooth movement distance gradually increased, and tooth movement distance in force group was higher than that in force+GsMTx4 group (P<0.05). RANKL, the ratio of RANKL/OPG and the number of osteoclasts in force group and force+GsMTx4 group showed a trend of increasing first and then decreasing, and OPG showed a gradual increasing trend. Compared with force group, the values in force+GsMTx4 group were all decreased (P<0.05). Conclusions: GsMTx4 can reduce the expression of RANKL and OPG proteins in the periodontal tissue at the pressure side during orthodontic tooth movement in rats, and inhibit osteoclasts differentiation, thereby reducing bone resorption activity and inhibiting tooth movement.

Key words: GsMTx4, orthodontic tooth movement, RANKL, OPG, Piezo1, osteoblast

References

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Effect of GsMTx4 on periodontal tissue reconstruction at the pressure side during orthodontic tooth movement in rats through RANKL/OPG pathway

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