Abstract: Objective:To explore whether TRPV1 in CeA of mice was involved in tooth movement pain and anxiety. Methods:Forty 8-week-old adult male C57BL/6J mice were randomly divided into blank group, 0 cN, 10 cN, 30 cN and 50 cN groups according to the tooth movement force. Then the behavioral indexes were recorded at different time points. The pain level of mice was evaluated by face wiping test, the anxiety-like state was evaluated by open field test and elevated maze test. Immunofluorescence method was used to observe the distribution and expression of TRPV1 in CeA, western blot method was used to detect the change trend of TRPV1 protein expression in mouse CeA during orthodontic tooth movement. Results:All the force values of mice showed pain during the experimental tooth movement, in which the 30 cN group showed the highest level of face wiping on the 1st day. The mice with different force values also showed different degrees of anxiety-like behavior, and the anxiety-like indexes in the 30 cN group were significantly higher than those in the 0 cN group on the 28th day (P<0.05). Immunofluorescence staining showed that the expression of TRPV1 in the 30 cN 1 d group was higher than that in the 0 cN 1 d group (P<0.001). Western blot experiment showed that the expression of TRPV1 in CeA in 30 cN group was significantly higher than that in 0 cN 1 d group during experimental tooth movement, and reached the peak on the 1st and 28th day (P<0.05). Conclusions:TRPV1 in CeA participated in pain and anxiety of experimental tooth movement.