原发性颞下颌关节色素沉着绒毛结节性滑膜炎的潜在诊断标志物预测研究

Abstract

Abstract: Objective:To identify potential diagnostic markers and assess their role in the pathogenesis and progress of temporomandibular joint (TMJ) pigmented villous nodular synovitis (PVNS) based on bioinformatics analysis. Methods:The gene expression profile was analyzed at the probe level according to the raw matrix data of gene chip (GSE3698). R language programming was used to analyze and visualize the results. DEGs were screened in TMJ PVNS. The functional annotation and signal pathway enrichment analysis on these cell populations. PPI network was established based on the STRING database. Summary receiver operating characteristic curves were plotted to determine diagnostic biomarkers of TMJ PVNS. Quantitative analysis of immune cell subtypes in TMJ PVNS was calculated using CIBERSORT software from sequences and the association between diagnostic markers and infiltrating immune cells was also estimated accordingly. Results:?Totally 139 DEGs were found out including 46 downregulated and 93 upregulated genes. Functional enrichment analysis revealed that 44 biological process, 30 cellular component, 18 molecular function, and 22 signal pathways were statistically significant. Eight hub genes with significantly upregulated expression were screened by PPI network. FCER1G, HLA-DPB1, LAPTM5, and TYROBP were identified as potential diagnostic biomarkers for TMJ PVNS. Immunocyte infiltration analysis showed neutrophils and M2 macrophages were associated with the pathogenesis and progress of TMJ PVNS. In addition, the correlation analysis concerning the identified diagnostic markers and infiltrating immune cell subpopulations indicated that FCER1G, LAPTM5, TYROBP were negatively related to plasma cell infiltration; FCER1G, HLA-DPB1, LAPTM5, and TYROBP were positively related to M2 macrophage infiltration and that neutrophils. Conclusions:This investigation concluded that FCER1G, HLA-DPB1, LAPTM5, and TYROBP as potential diagnostic markers and immune cell infiltration played an important role in the pathogenesis and progress of TMJ PVNS.

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Predictive study regarding potential diagnostic markers in primary pigmented villonodular synovitis of temporomandibular joint

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