Abstract: Objective:?Investigating whether apoptotic vesicles (apoVs) derived from stem cells from apical papilla (SCAP) modulate macrophage inflammatory phenotype by influencing glycolysis-associated enzymes, thereby regulating the inflammatory response. Methods:?Human-derived SCAP were cultured and characterized in vitro. Staurosporine induction was employed to obtain apoVs from SCAP, followed by the characterization and identification of the apoVs using scanning electron microscopy, flow cytometry, and other methods. Experimental groups included a blank control group, an inflammation group and an apoVs group. Rat bone marrow-derived macrophages (BMDMs) were treated with PBS, LPS and LPS in conjunction with apoVs. Using flow cytometry to assess the expression of surface markers CD80 and CD86 on pro-inflammatory macrophages; employing Western blot to detect the expression of glycolytic enzymes in macrophages. Results:?ApoVs derived from SCAP can be taken up by macrophages, reducing the expression of pro-inflammatory phenotype markers CD80 and CD86. Additionally, they exert an impact on glycolysis-associated enzymes, with a significant downregulation observed in the expression of GLUT1 and GLUT3 (P<0.05). Conclusions:?ApoVs derived from SCAP regulate the expression of glycolysis-associated enzymes in macrophages, reducing the distribution of pro-inflammatory phenotype macrophages, thereby modulating the inflammatory response.

Key words: stem cells from apical papilla, macrophages, apoptotic vesicles, glycolysis