Abstract: Objective: To compare the phagocytosis ability of Aa by peripheral mononuclear cells(Mo), alveolar macrophage(AM), peritoneal macrophage (PM) and kuffer cells (KC). Meathods: Mo, AM, PM and KC from 3 healthy New Zealand rabbits were isolated and purificated. Aa was labeded with fluorescein isothiocyanate (FITC). Monocytes/macrophages were infected with FITC-Aa in the ratio of 25:1(25 bacteria:1 cell). The phagocytosis rates and mean fluorescent intensity (MFI) were detected by the flow cytometry at 30 min, 1 h, 1.5 h. And phagocytic index was calculated. Result: Within 1.5 h, the AM, PM phagocytic index were rise as the time increase; the phagocytosis ability of Mo and KC reached the peak at 1 h; Obvious difference of phagocytosis between Mo, AM, PM and KC was observed. At 30 min, the phagocytic index is : AM and PM＞Mo, KC; at 1 h, AM＞PM＞Mo and KC; at 1.5 h, AM＞PM＞KC＞Mo. Conclusion: The different phagocytosis of Aa by monocytes/macrophages of various tissues may be the important reason why there are different effects on different parts in the periodontitis.