›› 2013, Vol. 4 ›› Issue (1): 19-22.

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Effects of platelet-rich fibrin on osteogenic differentiation of the rabbit adipose-derived stem cells

  

  • Received:2013-01-02 Revised:2013-03-08 Online:2013-03-25 Published:2013-03-30

Abstract: Objective:To Separate and cultivate rabbit adipose-derived stem cells (ADSCs) ,identify its differentiation activity and observe the effects of Platelet-rich fibrin(PRF)on the osteogenic differentiation of thoses cells. Methods:Iguinal fat tissue extracted from New Zealand rabbits and isolated adipose derived stem cells. The third generation ADSCs were used in the experiment. The methods of oil red O and alizarin red staining were carried out to identify the dipogenic and osteogenic differentiation .Blood collection was carried out from central artery of the rabbit and obtain the PRF membrane.The ADSCs were divided into two groups: the experiment group with a PRF membrane ;the control group without a PRF membrane.The alkaline phosphatase kit was used to observe the effect of PRF on the osteogenic differentiation of ADSCs.Results:The rabbit ADSCs grew in the way of long spindle adherence.The results of oil red O and alizarin red staining of the cells were positive. At different time points, the expression of alkaline phosphatase in the experiment group was significantly higher than the control group (p < 0.05). Conclusions:ADSCs can induced osteogenic differentiation and PRF can promote ADSCs to differentiate into osteoblasts.

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