Objective To investigate the role of BCL6 co-repressor, BCOR, on the myogenic differentiation potential of stem cells from apical papilla (SCAPs). Methods Retroviral full length HA-BCOR was used to over-express the BCOR in SCAPs; Western Blot was used to detect the expression of HA-BCOR; Recombinant human TGF-β1 protein was used to stimulate the myogenic differentiation in SCAPs; Real-time RT-PCR was used to detect the expressions of myogenesis related genes, Smoothened (SMO), actin, alpha 2, smooth muscle, aorta (ACTA2) and Caldeson (CALD1) to investigate myogenic differentiation capacity of SCAPs in vitro. Results Western Blot result showed that HA-BCOR could over-express in SCAPs; Over-expression of BCOR inhibited the expressions of SMO and CALD1 in SCAPs. Conclusion Our results indicated that over-expression of BCOR inhibited myogenic differentiation potential of SCAPs and BCOR was inhibitor for the myogenic differentiation in SCAPs.

Objective: To investigate the effect of Aa-LPS and IL-10 on apoptosis of Alveolar macrophages in vitro .Methods:Alveolar macrophages(AM) were harvested from the bronchoalveolar lavage fluid(BALF).These cells were randomly divided into three groups: control group, Aa-LPS group and Aa-LPS +IL-10 group. The number of cells was 1×106/ml in each group. Then these cells were given Aa-LPS(1μg/ml),IL-10（0.1μg/ml）.After 24 hours,the cells were harvested to extract RNA. Finally, RT-PCR was used to detect the expression of gene bax,p53 and caspase-3.Results:The results showed that the expression of bax and caspase-3 in the Aa-LPS group were significantly higher than the control group（P<0.05）.The expression of p53 was not statistical significant compared with the control group（P>0.05）. IL-10 treatment significantly inhibited macrophagocyte apoptosis and suppressed the expression of bax,p53 and caspase-3（P<0.05）.Conclusions: Aa-LPS can promote alveolar macrophages apoptosis in vitro and IL-10 can suppress the effect of Aa-LPS. The mechanism seemed related to the mitochondria-dependent pathway.

Objective：To construct the recombinant eukaryotic expression vector containing the recombination human cementum protein 1 (rhCEMP1) and to observe the expression of rhCEMP1 in yeast. Methods：RhCEMP1 cDNA was amplified by PCR, and correctly inserted into corresponding sites of intermediate vector pTeasy after restriction endonuclease digestion, and then inserted into corresponding sites of eukaryotic expression vertor pWX530 after another restriction endonuclease digestion. The recombinant vector pWX530-rhCEMP1 was confirmed to contain rhCEMP1 DNA sequence by agarose gel electrophoresis and DNA sequence analysis. The correct vector pWX530-rhCEMP1 was transfectd into yeast competent cells, the yeast was cultured after amino acid auxotroph screening. The expression of rhCEMP1 was determined by SDS-PAGE and enzyme-linked immunosorbent assay(ELISA),the rhCEMP1 was purified by ion exchange chromatography. Results： The recombinant plasmid was successfully transferred to the yeast cells. The rhCEMP1 was successful expressed by SDS-PAGE and ELISA analysis. Conclusions：The recombinant vector pWX530-rhCEMP1 was constructed successfully. The recombinant plasmid pWX530-rhCEMP1 could be transfected into yeast and expressed.

Abstract： Objective To study the expression of Golli-MBP (genes of the oligodendrocyte lineage-myelin basic protein，Golli-MBP) in lesions of oral lichen planus (OLP). Methods The mRNA expression level of Golli-MBP in 39 OLP tissues and 16 normal oral mucosa tissues was detected by real-time fluorescence quantitative PCR. The expression of Golli-MBP was also investigated in OLP (38 cases) and normal oral mucosa specimens (20 cases) using immunohistochemical technique. Results Increased Golli-MBP mRNA expression was found in OLP than that in normal oral mucosa(P<0.001). Expression of Golli-MBP in paraffin-embedded biopsy specimens was significantly higher in OLP than in normal controls(P<0.001); Golli-MBP expressed significantly higher in lymphocytes of OLP than in normal controls (P<0.05), while there were no significant differences in epithelial cells（P＞0.05）. Conclusions The data accumulated here indicate that the overexpression of Golli-MBP in OLP may play a role in the pathogenesis of OLP.

Objective: To identify the effects of dentin non-collagenous proteins (dNCPs) on the proliferation and mineralization of human dental pulp stem cells (hDPSCs) in vitro. Methods: hDPSCs were treated with 10 μg/mL dNCPs in normal medium for certain time periods. Proliferation of hDPSCs was evaluated by MTT colorimetric assay. Alkalinephosphotase (ALP) activity was determined by enzymekinetics methods. Alizarin red staining and cetylpyridinium chloride (CPC) assay were performed to investigate the mineralization ability of hDPSCs. Results: There was no difference between the proliferation activity of hDPSCs treated with dNCPs and the other group (P>0.05). ALP activity and mineralization ability of this group were signigicantly upregulated (P<0.01). Conclusions: 10 μg/mL dNCPs significantly promotes the mineralization of hDPSCs, while the proliferation ability shows no significant difference.

Objective：To Separate and cultivate rabbit adipose-derived stem cells (ADSCs) ,identify its differentiation activity and observe the effects of Platelet-rich fibrin（PRF）on the osteogenic differentiation of thoses cells. Methods：Iguinal fat tissue extracted from New Zealand rabbits and isolated adipose derived stem cells. The third generation ADSCs were used in the experiment. The methods of oil red O and alizarin red staining were carried out to identify the dipogenic and osteogenic differentiation .Blood collection was carried out from central artery of the rabbit and obtain the PRF membrane.The ADSCs were divided into two groups: the experiment group with a PRF membrane ;the control group without a PRF membrane.The alkaline phosphatase kit was used to observe the effect of PRF on the osteogenic differentiation of ADSCs.Results:The rabbit ADSCs grew in the way of long spindle adherence.The results of oil red O and alizarin red staining of the cells were positive. At different time points, the expression of alkaline phosphatase in the experiment group was significantly higher than the control group (p < 0.05). Conclusions：ADSCs can induced osteogenic differentiation and PRF can promote ADSCs to differentiate into osteoblasts.

[Abstract] Objective：To investigate the effect of parathyroid hormone- related protein (PTHrP)on the alveolar bone of ovariectomized rats. Methods：80 female 5-month-old rats were selected , among them 60 rats were ovariectomized (OVX) and the other 20 rats were sham-ovariectomized (sham-OVX) . Six weeks later the OVX rats were further divided into three groups randomly, each grouop consisting of 20 rats: the PTHrP treated group (PTHrP group) which was injected PTHrP，the estrogen treated group(E2 group) which was injected Estradiol benzoate , and the placebo group（OVX group）which was injected saline, while the 20 sham-OVX rats (sham-OVX group) were also injected saline as the blank control group. 60 days later, all the rats were sacrificed and their right mandibles were obtained for measuring bone mineral density (BMD) of molars region, besides, the alveolar bones of the first molars were dealt into tissue spices for histomorphological observation and immunohistochemical staining of Runt-related transcription factor 2 (Runx2). Results：The BMD measure indicated that the PTHrP group had a higher BMD value significantly than the OVX group（P＜0.05）, with no less than the E2 group or even the sham-OVX group（P＞0.05）. The histomorphological observation showed the morphology of alveolar bone of the PTHrP group approximated the E2 group and the sham-OVX group, without bone loss as apparent as the OVX group. Moreover, the analysis of immunohistochemical staining suggested that the Runx2 expression in PTHrP group was more than either of other groups, which showed significant statistical differences（P＜0.05）. Conclusions：PTHrP may have an anabolic effect on the alveolar bone of the ovariectomized rats.

[Abstract] Objective：To observe the resorption and healing process of replanted tooth in experimental rats, so we can assist the doctor to cure and prevent the root resorption in clinic. Methods: The experiment was carried out on thirty 6-week-old SPF Wistar male rats(n=5) ,the rats were randomly separated into six groups,and one of which is blank group. The bilateral maxillary first molars of each rat in experimental groups were extracted, randomly chose one molar to replant in the socket immediately, and the other one experienced delayed replantation. The rats were sacrificed on day 1, day 3, day 7, day 14 and day 21 after replantation. Then we segregated the maxillaes and scanned the specimens with X-ray. After decalcification, tissue slices were made and stained with HE. Results: The shadow area around the replanted tooth expanded over time, especially in the delayed replant group; the histological reaction of the tooth displayed that the resorption lacune around the root grew more and bigger with the developing inflammation, then the pulp tissue and periodontal ligament began to recover themselves in immediate replant group and aveolar bone recovered strongly with root and periodontal ligament insteaded by bone like tissue in delayed replant group. Conclusions: The reaction of replanted tooth showed mainly inflammation at first and recovery at last. The prognosis of replanted tooth mainly depend on the vitality of periodontal ligament cells.

Objective To study the effects of LiCl endogenous activation of Wnt/β-catenin signaling pathways of neure crest cells (NCCs) in mice tentacles mat area on NCCs proliferation. Methods Using immunofluorescence and western blot to observe the expression of β-catenin by 20mM/L LiCl for 24h. Using western blot to evaluate the expression of CyclinD1 and CyclinE1 in NCCs by LiCl for 24h. Using FCM to investigate the effects of different concentration and different time of LiCl on cell proliferation. Results The level of β-catenin, CyclinD1 and CyclinE1 were increased in NCCs. The strongest proliferation activity of NCCs took place after 24h by 20mM LiCl. Conclusions LiCl can activate Wnt/β-catenin signaling pathway and promote NCCs proliferation.

Objective: To investigate the normal development of mandibular condylar cartilage in the mice, and to clarify the change of RUNX expression and its meaning. Methods: Mandibular condyle of Balb/c mice were fixed from embryonic day 14.5 (E14.5) through Postnatal day 7.5(P7.5). Samples were cut at the sagittal plane. The histomorphology were examined using Hematoxylin-eosin staining(HE). The RUNX protein levels and distribution were detected using immunohistochemistry (IHC). Results: The mesenchymal cell aggregation began at E14.5, followed by the appearance of full-layer differentiated chondrocytes. The condylar morphology developed from semicircular into flat form. IHC showed the expressions of RUNX1, 2 focused in the proliferation, prechondrocyte and part of the hypertrophic layer, while the RUNX3 mainly expressed in the prechondrocyte and hypertrophic layer. The RUNX1, 2 expressions, both in condyles’ anterior (ant) and posterior (post) segments, were relatively higher in the prechondrocyte layer than the hypertrophic layer. The expression level of all RUNX presented as bimodal curve. Conclusion: The anterior (ant) and posterior (post) segment of condyle remained in premature stage, which suggested its remodeling potential. RUNX1 cooperated with RUNX2 in mediating the early stage of chondrogenesis. RUNX3 played an important role in chondrocyte maturation.

Objective：To investigate the histomorphological changes of the keratocystic odotogenic tumor after decompression. Methods：The histomorphologyical changes of 22 KCOT tissue slices stained with hematoxylin and eosin before decompression and after curettage were observed ，including the epithelial modality，the depth of the epithelium and connective tissue，the degree of inflammation infiltration adjective of the basal layer，then statistic analysis were made. Results：54.5 percent of the cases were found lost of epithelial para and localized hyperplasia with rete pegs, in addition, the thickness of both epithelium and connective layer increased statistically after decompression (P <0.05) and the degree of inflammation infiltration also intensified dramatically (P<0.05).Conclusions：The histomorphological features of KCOT have changed remarkably after decompression, appearing as the thickened linings and intensified inflammation infiltration, further research is needed to explain the mechanism of the above-mentioned changes.

Bioactive glass creates an environmental compatible with osteoconductive and osteoproductive, with the mineralizing interface developing as apatite layer between material and tissue. Recently, researches into bioactive glass and its application into dentistry has become the hotspot. The classification, bioactive characterization of bioactive glass and the application into dentistry are summarized.

Advanced glycation end products (AGEs ) are formed by anonenzymatic glycation of proteins, initiated by a reaction between reducing sugars and free amino groups in lysine or arginine residues,which play a important role in the pathogenesis of diabetes (Diabetic mellitus, DM )complications. Inflammatory bone disease is common complication of diabetes, The mechanism of AGE s in bone destruction of diabetes is not clear, This reviewed try to elaborate the effects of AGE s in diabetes inflammatory bone disease, development, and the influence of oateoclasts/osteoblasts。