›› 2013, Vol. 4 ›› Issue (1): 31-34.

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LiCl effects on proliferation of hair follicles nervous crest cells

  

  • Received:2012-08-30 Revised:2012-11-07 Online:2013-03-25 Published:2013-03-30

Abstract: Objective To study the effects of LiCl endogenous activation of Wnt/β-catenin signaling pathways of neure crest cells (NCCs) in mice tentacles mat area on NCCs proliferation. Methods Using immunofluorescence and western blot to observe the expression of β-catenin by 20mM/L LiCl for 24h. Using western blot to evaluate the expression of CyclinD1 and CyclinE1 in NCCs by LiCl for 24h. Using FCM to investigate the effects of different concentration and different time of LiCl on cell proliferation. Results The level of β-catenin, CyclinD1 and CyclinE1 were increased in NCCs. The strongest proliferation activity of NCCs took place after 24h by 20mM LiCl. Conclusions LiCl can activate Wnt/β-catenin signaling pathway and promote NCCs proliferation.

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