›› 2014, Vol. 5 ›› Issue (3): 120-124.

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Bacterial flora associated with the apical segment of teeth with persistent periapical periodontitis

  

  1. Affiliated Hospital of Stomatology, Nanjing Medical University
  • Received:2014-08-14 Revised:2014-08-27 Online:2014-09-25 Published:2014-09-24

Abstract: Objective:The aims of this study were to investigate bacterial flora associated with the apical segment of teeth with persistent periapical periodontitis. Methods: Apical root samples from root-end surgery were collected from 13 teeth with persistent periapical periodontitis. The DNA of the bacteria in 13 samples was processed for ampli?cation via polymerase chain reaction and separated with denaturing gradient gel electrophoresis. Selected bands were excised from the gel and sequenced for identi?cation. Results: All samples presented with several bands (strong and weak), which indicates a polymicrobial community. In our research, 26 excised bands were selected to identify the primary community members. The isolated strains consisted of facultative anaerobic bacteria, obligate anaerobic bacteria and aerobic bacteria. The prevalence rates of Actinomyces sp. oral and Propionibacterium were highest (84.6% and 61.5%, respectively). Conclusions:Bacterial floras associated with the apical segment of teeth with persistent periapical periodontitis are polymicrobial community. Actinomyces sp. oral and Propionibacterium are likely to be important contributors to persistent periapical infection.