›› 2014, Vol. 5 ›› Issue (3): 125-129.

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The radiosensitivity of cancer stem cells in squamous cell carcinoma of tongue

  

  • Received:2014-08-18 Revised:2014-09-11 Online:2014-09-25 Published:2014-09-24

Abstract: Objective: To investigate the radiosensitivity of cancer stem cells in squamous cell cacinoma of tongue. Methods:CD133+/CD44+ and CD133-/CD44- subpopulation cells were sorted by magnetic cell sorting (MACS). Single isolated CD133+/CD44+ and CD133-/CD44- cell was cultured in RPMI-1640 mediun,which contained 10 ng/mL basic fibroblast growth factor (bFGF),and 20 ng/mL epidermal growth factor (EGF). Immunofluorescence was applied to detect the expression of CD133 and CD44 in these two subpopulation cells. CCK-8 assay was performed to analyze the radiosensitivity of the two subpopulations. Results:Cell immunofluorescence showed that CD133 and CD44 could be detected in CD133+/CD44+ cells while they could not be detected in CD133-/CD44- cells. Radiation therapy had a more obvious inhibitory effect on CD133-/CD44- cells than on CD133+/CD44+ cells. Conclusions: The cancer stem cells in squamous cell carcinoma of tongue(CD133+/CD44+ cells) that sorted by MACS showed strong capability of tumor's resistance to radiation therapy than that of CD133-/CD44- cells.

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