CCL25/CCR9在口腔扁平苔藓外周血的表达及对T细胞功能的影响

Abstract

Abstract: Abstract:Objective:To explore the role of CCL25/CCR9 in pathogenesis of oral lichen planus (OLP). Methods： The expression of CCL25 in plasma and CCR9 of peripheral blood T cell of OLP were detected by qRT-PCR, WES and ELISA. Cultured T lymphocytes in vitro，the effects of CCL25/CCR9 on T cell migration, proliferation and apoptosis were detected by Transwell assay, CCK8 and flowcytometry. Results：The expression of peripheral blood T cell CCR9, CCL25 mRNA and CCL25 protein in plasma of OLP were decreased compared with those of the control group (P<0.01). CCL25 induces T cell to migrate, and the chemotactic index (CI) of OLP is lower than that of the control group (P<0.01). CI reduced significantly after blocked by anti-CCR9 mAb（P<0.05). Compared with the control group, the proliferation of T cell in peripheral blood of OLP was increased and the apoptosis was decreased, but they were not associated with the addition of CCL25 or anti-CCR9 mAb. Conclusions： CCL25 and CCR9 were lowly expressed in peripheral blood of OLP and their interaction can mediate T cell migrating. CCL25/CCR9 may be involved in recruitment of inflammatory cells during the pathogenesis of OLP.

Key words: Key words:CCL25/CCR9, OLP, T lymphocytes, migration, proliferation, apoptosis

References 13

[1]	Sagari S, Sanadhya S, Doddamani M, et al.Molecular markers in oral lichen planus: A systematic review[J].J Oral Maxillofac Pathol, 2016, 20(1):115-121
[2]	Wang H, Zhang D, Han Q, et al.Role of distinct CD4(+) T helper subset in pathogenesis of oral lichen planus[J].J Oral Pathol Med, 2016, 45(6):385-393
[3]	Zhang J, Romero J, Chan A, et al.Biarylsulfonamide CCR9 inhibitors for inflammatory bowel disease[J].Bioorg Med Chem Lett, 2015, 25(17):3661-3664
[4]	Wang C, Liu Z, Xu Zet al.The role of chemokine receptor 9/chemokine ligand 25 signaling: From immune cells to cancer cells[J].Oncol Lett, 2018, 16(2):2071-2077
[5]	Ferri EP, Gallo CB, Abboud CS, et al.Efficacy of photobiomodulation on oral lichen planus: a protocol study for a double-blind, randomised controlled clinical tria[J].BMJ Open, 2018, 8(10):e024083-
[6]	Hu JY, Zhang J, Cui JL, et al.Increasing CCL5/CCR5 on CD4+ T cells in peripheral blood of oral lichen planus[J].Cytokine, 2013, 62(1):141-145
[7]	Alvarez C, Benítez A, Rojas L, et al.Differential expression of CC chemokines (CCLs) and receptors (CCRs) by human T lymphocytes in response to different Aggregatibacter actinomycetemcomitans serotypes[J].J Appl Oral Sci, 2015, 23(6):580-590
[8]	Ding M, Zeng J, Sroussi H, et al.Interactions between Golli-MBP and Th1Th2 cytokines in patients with oral lichen planus[J].Oral Dis, 2014, 20(2):205-211
[9]	Hatzimanikatis V, Choe LH, Lee KH.Proteomics:theoretical and experimental considerations[J].Biotechnol Prog, 1999, 15(3):312-318
[10]	de Sousa Abreu R, Penalva LO, Marcotte EM, et al.Global signatures of protein and mRNA expression levels[J].Mol Biosyst, 2009, 5(12):1512-1526
[11]	Trivedi PJ, Bruns T, Ward S, et al.Intestinal CCL25 expression is increased in colitis and correlates with inflammatory activity[J].J Autoimmun, 2016, 68:98-104
[12]	雷蕾, 谭为霞, 周序珑，等.口腔扁平苔藓固有层淋巴细胞中Fas及Fas配体的表达变化[J].中华口腔医学杂志, 2010, 45(4):219-222
[13]	Shan J, Ma JM, Wang R, et al.Proliferation and apoptosis of peripheral blood mononuclear cells in patients with oral lichen planus[J].Inflammation, 2013, 36(2):419-425

Expression of CCL25/CCR9 in peripheral blood of oral lichen planus and its effect on T cell function

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