牙龈卟啉单胞菌诱导的耐受对小鼠牙槽骨吸收的影响

Abstract

Abstract: Objective: To investigate the effects of tolerance induced by Porphyromonas gingivalis (P.gingivalis) on bone resorption related cytokines, including IL-1β, osteoprotegerin (OPG)/receptor activator of NF-κB ligand (RANKL), and alveolar bone resorption in mice. Methods: Tolerance in murine periodontal tissues was induced by orally inoculation with 1×109CFU P.gingivalis for 5 days, washing with PBS and inoculation with P.gingivalis again for additional 2 days. After 10 days, fresh gingivae were collected and the expression levels of IL-1β, OPG and RANKL were detected by western bolt. 6 weeks later, maxillae were collected and the distances from the cementoenamel junction (CEJ) to the alveolar bone crest (ABC) were evaluated by methylene blue staining. Results: The expression levels of IL-1β in gingivae from P.gingivalis-tolerant mice were lower than those from non-tolerant group and the ratio of OPG/RANKL expression levels was higher than that of non-tolerant group. Less bone absorption was observed in tolerant group compared with that in non-tolerant mice (P<0.05). Conclusion:Tolerance induced by P.gingivalis could suppress

Key words: Porphyromonas gingivalis, tolerance, alveolar bone, resorption

References 17

[1]	Chung J H, Hwang H J, Kim S H, et al.Associations Between Periodontitis and Chronic Obstructive Pulmonary Disease: The 2010 to 2012 Korean National Health and Nutrition Examination Survey[J].J Periodontol, 2016, 87(8):864-871
[2]	Holden J A, Attard T J, Laughton K M, et al.Porphyromonas gingivalis lipopolysaccharide weakly activates M1 and M2 polarized mouse macrophages but induces inflammatory cytokines[J].Infect Immun, 2014, 82(10):4190-4203
[3]	Sirisinha S.Insight into the mechanisms regulating immune homeostasis in health and disease[J].Asian Pac J Allergy Immunol, 2011, 29(1):1-14
[4]	Soares-Schanoski A, Jurado T, Cordoba R, et al.Impaired antigen presentation and potent phagocytic activity identifying tumor-tolerant human monocytes[J].Biochem Biophys Res Commun, 2012, 423(2):331-337
[5]	Raju S M, Kumar A P, Yadav A N, et al.Haptoglobin improves acute phase response and endotoxin tolerance in response to bacterial LPS[J]. Immunol Lett, 2019, 207: 17-27.
[6]	Holcombe S J, Jacobs C C, Cook V L, et al.Duration of in vivo endotoxin tolerance in horses[J]. Vet Immunol Immunopathol, 2016, 173: 10-16.
[7]	Kramer C D, Genco C A.Microbiota, Immune Subversion, and Chronic Inflammation[J]. Front Immunol, 2017, 8: 255.
[8]	Petes C, Mintsopoulos V, Finnen R L, et al.The effects of CD14 and IL-27 on induction of endotoxin tolerance in human monocytes and macrophages[J].J Biol Chem, 2018, 293(45):17631-17645
[9]	Melo E S, Barbeiro H V, Ariga S, et al.Immune cells and oxidative stress in the endotoxin tolerance mouse model[J].Braz J Med Biol Res, 2010, 43(1):57-67
[10]	Baker P J, Dixon M, Roopenian D C.Genetic control of susceptibility to Porphyromonas gingivalis-induced alveolar bone loss in mice[J].Infect Immun, 2000, 68(10):5864-5868
[11]	Adhikari N, Neupane S, Aryal Y P, et al.Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice[J]. J Periodontal Res, 2019. doi: 10.1111/jre.12657. [Epub ahead of print]
[12]	Morita-Takemura S, Nakahara K, Hasegawa-Ishii S, et al.Responses of perivascular macrophages to circulating lipopolysaccharides in the subfornical organ with special reference to endotoxin tolerance[J].J Neuroinflammation, 2019, 16(1):39-
[13]	Yavuz M C, Pekbagriyanik T, Saglam M, et al.Evaluation of milk fat globule-epidermal growth factor-factor VIII and IL-1beta levels in gingival crevicular fluid and saliva in periodontal disease and health[J]. Odontology, 2019. doi: 10.1007/s10266-019-00419-5. [Epub ahead of print]
[14]	Hou L T, Liu C M, Liu B Y, et al.Interleukin-1beta,clinical parameters and matched cellular-histopathologic changes of biopsied gingival tissue from periodontitis patients[J].J Periodontal Res, 2003, 38(3):247-254
[15]	Longhini R, Aparecida D O P, Sasso-Cerri E, et al.Cimetidine reduces alveolar bone loss in induced periodontitis in rat molars[J].J Periodontol, 2014, 85(8):1115-1125
[16]	Kuhr A, Popa-Wagner A, Schmoll H, et al.Observations on experimental marginal periodontitis in rats[J].J Periodontal Res, 2004, 39(2):101-106
[17]	Souza D M, Prado F A, Prado M A, et al.Evaluation of two morphometric methods of bone loss percentages caused by periodontitis in rats in different locations[J].J Appl Oral Sci, 2010, 18(5):493-497

Tolerance Induced by Porphyromonas gingivalis Alleviates Periodontal Alveolar Bone Resorption in Mice

Knowledge

Abstract

Cite this article

share this article

References 17

Related Articles 7

Recommended Articles

Metrics

Comments

[1]	. MVP plays a protective role in periodontitis by inhibiting osteoclast differentiation and reducing bone resorption [J]. , 2020, 11(1): 24-27.
[2]	. Effect of platelet rich fibrin membrane on periodontal healing of delayed replanted rat tooth [J]. , 2017, 8(2): 95-99.
[3]	. Research progression of internal tooth resorption [J]. , 2016, 7(2): 90-92.
[4]	. Experimental research into the influence of two kinds of storage media on the replantation of avulsed tooth [J]. , 2014, 5(2): 85-90.
[5]	. Effects of parathyroid hormone- related protein on the alveolar bone of ovariectomized rats [J]. , 2013, 4(1): 23-26.
[6]	. Dynamic observation of root resorption followed tooth replantation [J]. , 2013, 4(1): 27-30.
[7]	. Study on the correlation between red bacteria complex and retrograde pulpitis [J]. , 2012, 3(1): 13-16.