主穹隆蛋白通过抑制破骨细胞分化在牙周炎中起骨保护作用

Abstract

Abstract: Objective: To investigate the role of major vault protein (MVP) in periodontitis by establishing an experimentalmodel of periodontitison mice and osteoclast induction of bone marrow mesenchymal stem cells (BMMSCs) in vitro. Methods:A model of experimental periodontitis was established by local injection of lipopolysaccharide (LPS) into wild-type mice (WT) and MVP knockout (MVP-/-) mice.The severity of periodontitis was detected by micro-CT and TRAP staining. In vitro, the role of MVP in osteoclast differentiation was verified by TRAP staining and Wheat Germ Agglutinin (WGA) staining after cultured with RANKL and M-CSF. Results:In experimental periodontitis induced by LPS, MVP-/- group developed more bone resorption and more osteoclasts could be seen in the injection area. In vitro experiments showed that knockout MVP promoted osteoclast differentiation and bone resorption.Conclusions:MVP plays a protective role in periodontitis by inhibiting osteoclast differentiation.

Key words: MVP, osteoblast, differentiation, bone resorption

References 20

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MVP plays a protective role in periodontitis by inhibiting osteoclast differentiation and reducing bone resorption

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