›› 2020, Vol. 11 ›› Issue (1): 19-23.

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Age-related changes of proteoglycans during fracture healing

  

  • Received:2019-11-01 Revised:2020-03-05 Online:2020-03-25 Published:2020-04-07

Abstract: Objective: To investigate the role of age-related proteoglycans in fracture healing by establishing a model of femur fracture in mice. Methods: We established right femur fracture models on 2 months and 9 months C57BL/6J, they were sacrificed at 7 days post surgery. The fracture healing was observed by histological stain, the expression of chondrogenic genes and proteoglycan was detected by Real-time PCR. Results: The mouse femur fracture model was successfully constructed. Histological staining showed that the callus of 9 months mice was smaller than that of 2 months mice after fracture. Real-time PCR showed the expression of cartilage-related genes such as Col10, Sox9, Mmp9 and proteoglycan-related genes such as Dmp1, Vcan, Dcn, Bgn during fracture healing of 9 months mice were lower than that of 2 months mice (P<0.05). Conclusion: In the process of fracture healing, the expression of proteoglycans in aged mice is decreased, suggesting that proteoglycans may be related to the poor fracture healing in aged mice.

Key words: Bone fracture, Aging, Proteoglycan, Cartilage