硫化氢促进炎症微环境中正畸力作用下的牙周组织成骨分化

Abstract

Abstract: Objective:This study investigated the effects of H2S on orthodontic bone formation in rats with periodontitis and osteogenic differentiation of human periodontal ligament cells (hPDLCs) in the inflammatory microenvironment. Methods:Forty-eight rats were divided into four groups: phosphate-buffered saline control group, orthodontic treatment (OT) group, ligature induced-periodontitis+OT group, ligature induced-periodontitis+OT with NaHS (H2S donor) group. Bone formation at tension sites of periodontal tissue was observed by micro-computed tomography (micro-CT), immunohistochemistry staining for osteocalcin (OCN) and alkaline phosphatase (ALP) staining. Expression of osteogenesis related factors were examined by real-time quantitative PCR. In vitro, experiments were divided into control group, tension (T) group, lipopolysaccharide+T group, lipopolysaccharide+T with NaHS group. Real-time quantitative PCR was used to detect the expression of hPDLCs osteogenesis-related factors under tension force in the inflammatory environment. Results:It was found that the bone tissue parameters of the tension side in the P+OT+NaHS group were higher than those in the P+OT group in vivo. Compared with the P+OT group, the expression of Col1, Alp and Ocn osteogenesis-related genes and proteins were significantly increased in the P+OT+NaHS group（P<0.05）. At the same time, it was showed that the LPS+T+NaHS group, compared with the LPS+T group, had deeper staining results, higher OD value and significantly increased expressions of COL1, ALP and OCN osteogenesis-related genes in vitro（P<0.05）. Conclusions:NaHS could maintain bone mineral density in periodontal tissue, up-regulate the mRNA expression of osteogenic factors inhibited by inflammation, and promote orthodontic force-induced bone formation in an inflammatory microenvironment. Our findings suggested that H2S may play an important role in promoting bone formation of orthodontic teeth in periodontitis environment.

CLC Number:

R781.4

References 25

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Hydrogen sulfide improves orthodontic force-induced osteogenesis of periodontal tissue in inflammatory microenvironment

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