白藜芦醇通过抑制铁死亡保护骨髓间充质干细胞

Abstract

Abstract: Objective:?To explore the protective effect of Res on BMSCs under oxidative stress in vitro. Methods:? Rat BMSCs were treated with 200 μmol/L hydrogen peroxide to undergo oxidative stress. CCK8, ROS fluorescent probe, qRT-PCR, Western blot and ALP staining examined the effects of different concentration of Res on the cell viability, intracellular ROS levels, osteogenic differentiation and ferroptosis in rat BMSCs under oxidative stress. Results:?Res between 1 and 25 μmol/L promoted the cell viability of rat BMSCs stimulated by hydrogen peroxide (P<0.05). 10 μmol/L Res significantly reduced the ROS levels of hydrogen peroxide -stimulated rat BMSCs and increased the expression of antioxidant-related genes, such as NRF2, CAT, SOD-1 and SOD-2 (P＜0.05). 10 μmol/L Res promoted the expression of osteogenic-related markers of hydrogen peroxide -stimulated rat BMSCs (P＜0.05), significantly enhanced the ALP staining effect, promoted the expression of ferroptosis-related markers (GPX4, XCT) (P＜0.05), decreased the expression of acyl-CoA synthetase long-chain family member(ACSL4), prostaglandin-endoperoxide synthase 2 (PTGS2) and nuclear receptor coactivator 4 (NCOA4) (P＜0.05). Conclusions:?Res could reduce the level of ROS and ferroptosis of oxidative stress-induced rat BMSCs, maintain the cell viability and promote the osteogenic differentiation of rat BMSCs under oxidative stress.

Key words: Resveratrol, oxidative stress, osteogenic differentiation, ferroptosis

References

[1]. Khosla S, Hofbauer LC. Osteoporosis treatment: recent developments and ongoing challenges. Lancet Diabetes Endocrinol. 2017;5(11):898-907.
[2]. Ebeling PR, Nguyen HH, Aleksova J, et al. Secondary Osteoporosis. Endocr Rev. 2022;43(2):240-313.
[3]. Atashi F, Modarressi A, Pepper MS. The role of reactive oxygen species in mesenchymal stem cell adipogenic and osteogenic differentiation: a review. Stem Cells Dev. 2015;24(10):1150-1163.
[4]. Bartell SM, Kim HN, Ambrogini E, et al. FoxO proteins restrain osteoclastogenesis and bone resorption by attenuating H2O2 accumulation. Nat Commun. 2014;5:3773.
[5]. Deng S, Dai G, Chen S, et al. Dexamethasone induces osteoblast apoptosis through ROS-PI3K/AKT/GSK3beta signaling pathway. Biomed Pharmacother. 2019;110:602-608.
[6]. Yang F, Yang L, Li Y, et al. Melatonin protects bone marrow mesenchymal stem cells against iron overload-induced aberrant differentiation and senescence. J Pineal Res. 2017;63(3).
[7]. Park E, Chung SW. ROS-mediated autophagy increases intracellular iron levels and ferroptosis by ferritin and transferrin receptor regulation. Cell Death Dis. 2019;10(11):822.
[8]. Ma H, Wang X, Zhang W, et al. Melatonin Suppresses Ferroptosis Induced by High Glucose via Activation of the Nrf2/HO-1 Signaling Pathway in Type 2 Diabetic Osteoporosis. Oxid Med Cell Longev. 2020;2020:9067610.
[9]. Galiniak S, Aebisher D, Bartusik-Aebisher D. Health benefits of resveratrol administration. Acta Biochim Pol. 2019;66(1):13-21.
[10]. Zhu SY, Zhuang JS, Wu Q, et al. Advanced oxidation protein products induce pre-osteoblast apoptosis through a nicotinamide adenine dinucleotide phosphate oxidase-dependent, mitogen-activated protein kinases-mediated intrinsic apoptosis pathway. Aging Cell. 2018;17(4):e12764.
[11]. Agidigbi TS, Kim C. Reactive Oxygen Species in Osteoclast Differentiation and Possible Pharmaceutical Targets of ROS-Mediated Osteoclast Diseases. Int J Mol Sci. 2019;20(14).
[12]. Mohsin S, Baniyas MM, AlDarmaki RS, et al. An update on therapies for the treatment of diabetes-induced osteoporosis. Expert Opin Biol Ther. 2019;19(9):937-948.
[13]. Ensrud KE. Bisphosphonates for Postmenopausal Osteoporosis. JAMA. 2021;325(1):96.
[14]. Yang G, Chang CC, Yang Y, et al. Resveratrol Alleviates Rheumatoid Arthritis via Reducing ROS and Inflammation, Inhibiting MAPK Signaling Pathways, and Suppressing Angiogenesis. J Agric Food Chem. 2018;66(49):12953-12960.
[15]. Boissy P, Andersen TL, Abdallah BM, et al. Resveratrol inhibits myeloma cell growth, prevents osteoclast formation, and promotes osteoblast differentiation. Cancer Res. 2005;65(21):9943-9952.

Resveratrol protects bone marrow mesenchymal stem cells by inhibiting ferroptosis

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	. Effect of silencing PGC-1α on the directional differentiation of human stem cells from apical papilla [J]. Oral Biomedicine, 2023, (3): 146-150.
[2]	. Feather keratin-montmorillonite nanocomposite hydrogel on the biological behavior and osteogenic properties of bone marrow mesenchymal stem cell [J]. Oral Biomedicine, 2023, 14(2): 105-112.
[3]	. Effect and mechanism of calcium hydroxide paste on rat odontogenic / osteogenic differentiation of BMMSCs [J]. Oral Biomedicine, 2023, 14(2): 74-79,96.
[4]	. Effect of PLA/PVP-GEL composite scaffold on the biological behavior and osteogenic properties of MC3T3-E1 cell [J]. Oral Biomedicine, 2022, 13(4): 234-240.
[5]	. Experimental study about the effects of miR-431-5p on the proliferation and differentiation of human dental pulp stem cells by targeting Smad4 [J]. Oral Biomedicine, 2022, 13(4): 229-233.
[6]	. Effect of grafting chlorogenic acid-chitosan complex on zirconia implant surface on osteointegration [J]. Oral Biomedicine, 2022, 13(3): 142-147.
[7]	. Ferroptosis of oral squamous cell carcinoma induced by ferrocenyl retinoic acid nanoparticles [J]. Oral Biomedicine, 2022, 13(3): 165-168,174.
[8]	. Effects of polygonatum sibiricum polysaccharide on proliferation and osteogenic differentiation of stem cells from human exfoliated deciduous teeth [J]. Oral Biomedicine, 2022, 13(1): 40-44.
[9]	Xinquan 无Jiang. Magnesium-doped TiO2 coating on titanium by miro-arc oxidation promoting osteogenic differentiation of rat BMSCs [J]. Oral Biomedicine, 2021, 12(2): 76-81.
[10]	. The preliminary study of CDs for tracing and promoting osteogenic differentiation of rat BMSCs [J]. Oral Biomedicine, 2021, 12(1): 5-11.
[11]	. The regulation of miR-21 in inflammatory response and osteogenic differentiation of periodontal ligament stem cells [J]. , 2020, 11(2): 86-91.
[12]	. Research advances in alleviating the senescence of mesenchymal stem cells [J]. , 2020, 11(2): 131-136.
[13]	. The effect of stiffness of matrix on the osteoblasts growth and differentiation [J]. , 2020, 11(1): 13-18.
[14]	. Effects of miR-9 on the function of human periodontal ligament stem cells [J]. , 2019, 10(3): 114-117.
[15]	. Effect of electrospun SF/CS composite fiber scaffold on cell proliferation and osteogenic differentiation of hBMSCs in vitro [J]. , 2019, 10(2): 73-82.