结缔组织生长因子对NELL-1诱导的MC3T3-E1细胞成骨分化的影响

Abstract

Abstract:

Objective: This study aims at exploring the role of connective tissue growth factor (CTGF) on Nel-1 like molecule type 1(NELL-1)-induced osteogenesis in MC3T3-E1 cells. Methods: The MC3T3-E1 cell lineage was used in the study, which was treated with each of control (PBS), NELL-1 alone, CTGF alone or in combination of NELL-1 and CTGF. Then cell proliferation was employed with help of CCK-8 assay. After osteogenic induction, multiple experimental techniques including alizarin red staining with quantitative analysis, ALP activity assay and ALP staining, quantitative real time poly chain reaction (qRT-PCR) and western blot were used to detect cell osteogenic differentiation among each group. Results: Compared to control, CTGF partly inhibited MC3T3-E1 cell proliferation; while NELL-1 treatment alone induced more mineralized nodules formation, promoted ALP activity and darker ALP staining in MC3T3-E1 cells with increased transcription and expression levels of osteogenic genes, such as RUNX2, ALP and OSX (P<0.01). However, compared to NELL-1 treatment alone, less mineralized nodules formation, decreased ALP activity and light ALP staining with dramatically down-regulated transcription and expression of RUNX2, ALP and OSX were found in cells treated with in combination of NELL-1 and CTGF (P<0.05). Conclusions: CTGF inhibited NELL-1-induced osteogenesis in MC3T3-E1 cells.s.?

Key words: connective tissue growth factor (CTGF), nel-like molecule type 1(NELL-1), osteogenesis

References

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The effect of connective tissue growth factor on NELL-1-induced osteogenesis in MC3T3-E1 cells

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