Abstract: The cross-dermal interaction and secretion of related signaling molecules drived cell differentiation and organogenesis. From pattern formation, morphogenesis, to functional differentiation of teeth, various cross-dermal signaling molecules were involved. In recent years, with the application of novel technologies such as single-cell transcription profiling, some unique cell subpopulations and intercellular interactions have been reported, promoting further research on tooth development. This article reviews new findings in the use of single-cell transcriptome sequencing to study tooth development processes in recent years, with a focus on advances in cross-dermal intercellular signaling.

Key words: single-cell RNA sequencing, tooth development, signaling molecules, stem cell