Abstract: Objective:?To preliminarily screen and analyze the differentially expressed long non-coding RNAs in human parotid pleomorphic adenoma. Methods:?Three pairs of human parotid pleomorphic adenoma tumors (tumor group) and adjacent specimens (control group) were collected and differentially expressed lncRNAwere detected by high-throughput lncRNA-chip. By calculating the Pearson correlation coefficient (pcc), lncRNA co-expressed with pleomorphic adenoma gene 1 (PLAG1) and catenin beta 1 (CTNNB1) were screened, and the expression of interested lncRNA were verified using real-time PCR. Results: Compared to the control group, a total of 17382 lncRNA showed significant differencesin human parotid pleomorphic adenoma tumor samples; among these lncRNA, 8182 were up-regulated, and 9200 were down-regulated, which were mainly anti-sense and intergenic lncRNA (P＜0.05). Meanwhile, 23 lncRNA co-expressed with PLAG1 and CTNNB1 were screened (11 upregulated and 12 downregulated), among which ENST000000609511 showed no statistical difference in following validation (P＞0.05), while the expression of T256768 in tumor samples was significantly reduced compared to the control group (P＜0.05). Conclusions:?The differentially expression of lncRNA-T256768 may be related to the occurrence and development of parotid pleomorphic adenoma.

Key words: Pleomorphic adenoma, parotid gland, salivary gland tumor, long non-coding RNA, PLAG1