Objective：To prepare chitosan thermosensitive hydrogel for slow release both baicalin and bovine serum albumin (BSA), and to observe the release properties vitro. Methods：The baicalin-gelatin microspheres (GMS) were prepared by chemical emulsion polycondensation. The chitosan thermosensitive hydrogel system was prepared by using different proportions of chitosan solution and β-GP, observed gelation at 37℃, and selected the best ratio. On this basis, the different concentrations of baicalin-GMS and BSA were incorporated into the chitosan thermosensitive hydrogel system. Results: Baicalin-GMS were prepared successfully, and the rate of drug loading and encapsulation efficiency was respectively 5.62% and 72.05%. The ideal state of the gel was formed by 1.8% chitosan solution and 9% after 10 min. When two drugs were incorporated into chitosan thermosensitive hydrgel, There was no apparent change in the time of phase transition of gel solution. The low concentration group cumulative release was 63.79% afer 30 days and the two higher concentrations were released 74.86%, 77.63%. Conclusions：Chitosan thermosensitive hydrogel system can simultaneously loaded baicalin-GMS and BSA, melt at room temperature. It can be transformed into semi-solid gel at 37℃ after 10 min, and drug release up to more than 30 d in vitro.

Objective：To design a new tooth notation system to record teeth efficiently with computer for electronic patient records, electronic communication and electronic publications. Methods：T and t were considered as identification characters of teeth to be recorded. T represented permanent teeth and t represented deciduous teeth. From the midline posteriorly digits 1～8 and digits 1～5 represented the names of permanent teeth and deciduous teeth respectively. The assigned digits were written as superscript and subscript on right and left sides of characters. Results：The location and name of tooth in the dentitions can be recorded accurately by writing the assigned digit as superscript or subscript on right or left side of character. Conclusions：Teeth can be denoted by superscript-subscript tooth notation system conveniently and accurately.

Objective：To establish an animal model of immediate tooth replantation and make further study of the behaviors of healing and absorption in the replanted tooth. Methods：The experiment was carried out on thirty 6-week-old SPF Wistar male rats(n=5). The unilateral maxillary first molar of each rat were extracted and replanted immediately, nothing was done on the other side which was the control side. The rats were randomly separated into six groups and were sacrificed on day 1, day 3, day 7, day 14, day 21 and day 28 after replantation. After sacrificing the rats, we segregated the maxillaries and scanned the samples with X-ray, so as to analyze the shaded area around the mesial root of each tooth with the Image Pro Plus software. At last tissue slices were made and stained with HE, in order to obtain histological results. Results：Radiographers displayed that the areas of root resorption expanded over time. Histologic feature showed that the number of lacuna on root surface increased with time, the width of the periodontal ligament decreased and the number of new bone like tissue increased. Conclusions：The animal model of rat tooth replantation were successfully established, and it was stable and reliable. This model reflected the healing ways and developmental resorption process of replanted tooth completely. It has been provided a good experimental model for the further research of the molecular mechanism of replanted tooth healing.

Objective：To determinate the production of farnesol which is secreted by Candida albicans biofilms in different medium. Methods：Candida albicans SC5314 were vaccinated in RPMI1640, YPD and RPMI1640+10%FCS medium to form biofilms. At 1.5, 3, 6, 12, 24 and 48 h, farnesol was detected by gas chromatography-mass spectrometry. Results：In RPMI1640 and YPD medium, farnesol gradually increased with the biofilms development. Between the late stage of biofilms development and the mature stage, the production achieved to a high peak. In RPMI1640+10%FCS medium, farnesol gradually increased in the period of 48 hours. At 12 h, the production of farnesol in YPD was more than the others（P＜0.05）. At 48 h, the production of farnesol in RPMI1640+10%FCS was the highest（P＜0.05）. Conclusions：The medium play an important role in the production of farnesol by Candida albicans biofilms.

Objective：To observe the changes in enamel of rat incisor under chronic fluorine poisoning，and establish a new index for evaluation of dental fluorosis in rat. Methods：20 Wistar rats were randomly divided into four groups by gender. Control groups were divided into male and female group, fed with double-distilled water. Fluorosis groups were fed with double-distilled water containing 100 mg/L of F﹣ for 8 weeks. Under natural light, the changes of the mandibular incisor enamel were recorded by digital camera. The progress of dental fluorosis was evaluated by an improved dental fluorosis index. Results：Compared with control group, the enamel of dental fluorosis changed in different degrees, such as clarity, glossiness, surface defects. The progress of dental fluorosis had a significantly increased by drinking fluoride time. Under the same condition, dental fluorosis index of the different genders had no significant difference in sex（P > 0.05）. Conclusions： The improved dental fluorosis index can be used to evaluate the severity of dental fluorosis in rats objectively.

Objective：To investigate the relationship between the gene BGL2, XOG1 and Candida albicans biofilm drug resistance. Methods：Candida albicans drug resistance was induced by Fluconazole with gradually increased concentration，and identified by the KONT MIC determination system. RT-PCR and qRT-PCR were used to analyze the expressions of glucan-related genes in Candida albicans SC5314 and the drug resistant strain. Results：The KONT MIC determination system showed the minimal inhibitory concentration (MIC) of the drug resistant strain was more than 128 μg/ml. Compared with the control strain, the expression of XOG1 in the drug resistant strain was significantly reduced（P <0.05）, but the expression of BGL2 was not significantly changed. Conclusions：Glucan-related gene XOG1 was related to the resistance of Candida albicans biofilm.

Objective：To assess the anti-microbial effect of Polident denture cleaning tablets. Methods：International standard strains were selected and cultured by using in vitro methods with quantitative suspension test. Results：That denture-cleaning tablets (Polident) solution at 40 ℃, 5 minutes on Streptococcus sanguis, Actinomyces viscosus, Klebsiella pneumoniae, Fusobacterium nucleatum, Veillonella atypica, Candida albicans, Streptococcus mutans, Porphyromonas gingivalis, Prevotella intermedia, Pseudomonas aeruginosa, Streptococcus pneumoniae had 99.9% of bacteria sterilization. Conclusions：Polident denture cleaning tablets for some oral bacteria have permanent antimicrobial effect.

To detect the influences of saliva contamination on the bond strengths of glass ionomer cement (Ketac, 3M), self-adhesive universal resin cement (RelyX, 3M) and self-etching adhesive systems (Contax, DMG). Methods：Artificial saliva was made. Human third molars were collected and 90 test specimens were made and divided into 6 groups according to three different cements and immerged into artificial saliva or not. The microtensile bond strength was measured. Results：In the control and contamination groups, microtensiles of RelyX and Contax cements groups were higher than that of Ketac groups. Microtensiles of 0 min and 3 min groups reduced more obviously than that of 6 min and 10 min which were not significantly different from control groups. Conclusions：Saliva contamination can influence the bond strengths of three cements in the early period of solidification. Bond strengths of glass ionomer cement reduce more than that of composite resin cement.

Abstract：Demineralized bone matrix (DBM) is a product which is created through demineralized, degreased and deproteinized of allograft or xenograft. DBM is mainly comprised of collagen and bone morphogenetic proteins (BMPs). It has satisfied biocompatibility and excellent three-dimensional space and can stimulate local undifferentiated mesenchymal cells to transform into osteoblasts or Chondroblasts, this is important for promoting the formation of bone and cartilage. This material has been used in clinical for the treatment of bone defects and shows good results. The purpose of this paper is to review the mechanism of the DBM in repairing bone defects, oral maxillofacial applications and to prospect the feasibility applications of DBM in dental pulp repair.

Facial asymmetry is one of the most common diseases, which has a profound influence on people’s health, both physically and mentally. With the trend of pursuing beauty ascending, we feel the urgent need to get an insight into it. Due to the relatively dispersed researches earlier, this article summarizes the recent research progresses of the incidence rate of deformity, etiology, location, classification and treatment for the facial asmmetry.

The clinical application of mini-implant in dentistry. LI yao-qin ,Hu Jian（Institute of Stomatology,Nanjing Medical University,Nanjing 210029 China） Abstract:With the rapid development of implant，the mini-implant has obtained good clinical results, which led to expanded use in orthodontic anchorage, and long term fixed and removable prosthrtics.This article reviews details of backgound,the clinical application，the complications and the prospect.

Periodontitis is induced by bacterial biofilm that forms on teeth characterized by irreversible destruction of periodontal tissue. However, the inflammatory response to infection is influenced by environmental factors as well as by genetic factors. This review focused on the research advances of genetic characteristics of periodontitis, the related gene polymorphisms, and the genetic family diseases which increases periodontitis susceptibility.