Objective：To construct the pQCXIH-HA-FBXL11 retroviral expression plasmid, identify the expression, and establish stable cells that over-expressed FBXL11 in mesenchymal stem cells (MSCs). Methods：PCR was used to amplify the target gene, FBXL11, using cDNA of the dental pulp stem cells as a template. PCR products were purified and recycled, ligation with appropriate vector. Enzyme digestion and sequencing were used to identify the pQCXIH-HA-FBXL11 plasmid. The plasmids were transfect in 293T cells using FuGENE6, Western blot and real-time PCR were used to detect the expressions of FBXL11 at the protein and mRNA levels level. Retroviruses were infected the dental pulp-derived mesenchymal stem cells (DPSCs). Results：We successfully constructed the retroviral plasmid of pQCXIH-HA-FBXL11, confirmed its expression in 293T cells by Western blot and real-time PCR, and successfully established the FBXL11 over-expressed stable cells in DPSCs. Conclusions：The construction and expression of retroviral pQCXIH-HA-FBXL11 and over-expressed FBXL11 in DPSCs were the basis to investigate the function of FBXL11 in mesenchymal stem cells.

Objective：To construct specific miRNA expression vectors targeting membrane-type matrix metalloproteinase-1（MT1-MMP）gene and test the silent rates of four different vectors for MT1-MMP. Methods：Design four different specific oligo sequences of DNA targeting MT1-MMP, and then construct recombinant plasmids using BLOCK-iT? Pol II miR RNAi Expression Vector Kit with EmGFP. The positive ones were cloned. The transformed clones were sequenced and then amplified. The resultant correct plasmids were extracted and then transfected into the HEK293 cells by Lipofectamine 2000 to test the silent rates of MT1-MMP with real-time fluorogenic quantitative RT-PCR method．Results：The MT1-MMP miRNA interference vectors were constructed. The most effective interference vector was X173 - 4, and its target gene silencing efficiency can reach 75%. Conclusions：This study provides an experimental basis for further study on the role of MT1-MMP in invasion and metastasis of oral squamous cell carcinoma.

Objective：To study the effect of FOXM1 gene on proliferation of salivary adenoid cystic carcinoma cells. Methods：The highly metastatic adenoid cystic carcinoma cell line (SACC-M) was transient transfected by constructing FOXM1 siRNA interference clips. The interference efficiency was examined by Western blotting. Cell cycle changes were determined with flow cytometry. Cell proliferation was evaluated by MTT. The protein expression of CyclinB, CDC2 was examined by Western blotting. Cell migration was examined by wound-healing assay. Results：FOXM1 siRNA transfection specifically down-regulated the FOXM1 protein in SACC-M cells. Proliferation of SACC-M cells was suppressed，and the percentage of S stage cells was significantly reduced. Cells migration did not show a clear change. Conclusions：FOXM1 could regulate cell proliferation of salivary adenoid cystic carcinoma cells, but had no obvious effects on cell migration.

Objective：To study the biocompatibility of a new kind of biodegradable poly butylene carbonate (PBC) electrospun film. Methods：Polycaprolactone (PCL) electrospun membranes were prepared as control. Contact angles on the surfaces of two kinds of films were tested. Cell line MC3T3-E1 was seeded onto both kinds of membranes. SEM images were taken to characterize the original morphology of electrospun films and cells one day after seeding. MTT and ALP tests were performed to test the influence of electrospun films on proliferation and differentiation of tested cells. Results：Contact angles in PBC group were lower than those in PCL group, and the difference was significant (P<0.05). One day after seeding, cells attached tightly with both PBC and PCL membranes and spread well on them. There were no significant differences in effects on cell proliferation between two kinds of membranes on the 1st and 7th day (P>0.05), while ALP values in PBC group were significantly higher than those in PCL group on the 7th day (P<0.05). Conclusions：Compared with PCL films, PBC films were a little better in hydrophilicity and cell differentiation, and can be further studied for biomedical use.

Objective：To investigate the effects of SFCS nerve conduits on repairing facial nerve defect in rabbits by transplantation. Methods：Twenty-one male rabbits were made into models with 8 mm gaps on both sides of buccal branches of the facial nerve. SFCS nerve conduits were used to repair the right gaps of facial nerve, regarded as the experimental group. Autologous nerve were grafted into the left gaps, regarded as the control group. Electrophysilogical examination of nervous system was performed 4, 6, 8 weeks after operation, and general observation was done with microscope 2,4,6,8 weeks after operation. HE and Toluidine blue staining histological examination were performed, and image analysis system was used to analyze images. Results：The conduction velocity of the facial nerve﹝(27.50±3.00)m/s﹞, the counts of axon per unit area(741.80±49.92), and the thickness of the myelin sheath﹝(12.71±0.42)pm﹞of rabbits in experimental group were not statistically different with those in control group（P＞0.05）. Conclusions：The SFCS nerve conduits can effectively promote axon regeneration of rabbit facial nerve. They can potentially be used as a substitute for autografts to repair nerve defects.

Objective：To investigate the expression of apoptosis-associated protein Survivin in human odontogenic keratocyst (OKC) and its relationship with the expression of Caspase-3 and p53. Methods：TUNEL was used to detect the distribution of apoptotic cells in OKC. Immunohistochemical assay was used to detect the expression of Survivin, Caspase-3, p53 proteins in 40 cases of OKC ( including 20 cases of primary OKC, 20 cases of recurrent OKC). Results：Apoptotic cells were mainly seen in cells of surface layer of OKC epithelial lining . Survivin positive staining was mainly in basal and suprabasal layer cells of 26（65%）cases, Caspase-3 positive staining were mainly in surface epithelial cells of 18（45%）cases. The expression of p53 was negative in OKC of all cases. Conclusions：Apoptosis-associated protein Survivin and Caspase-3 play an important role in generation and development of OKC.

Objective：To investigate effects of osteoporosis on osteointegration of implants in tibias of rats. Methods：Twenty old female Wistar rats were randomly divided into two groups: Sham（control group）and OVX （model group）. The serum alkaline phosphatase (ALP) activity was measured before and 3 months after operation. Shin bone mineral density and HE staining were examined 3 months after operation. One implant was inserted in a tibia of each rat in Sham and OVX groups. Four and eight weeks after implantation, tibias with implants were taken pictures of by X-ray. The osteointegration of bones and implants in each group were observed by histological slices and SEM scanning. Results：Three months later, tibias showed decreased bone density and trabecularism porosity enlargement in OVX group, and the serum ALP activity of OVX group was significantly higher than that before operation( P＜0.05)，but no change was found in Sham group. As a result, models of osteoporosis were successfully set up in OVX group. New bone formation was determined in peri-implant area with good osteointegration in Sham group 4 weeks and 8 weeks after implantation. The new cementum was like bone cortex, and it became gomphosis with spiricle of implants.　No obvious gap was observed. The osteointegration went on well. In OVX group, a little new bone formation was found in partial peri-implant area, the structure was irregular and it was far from implants. Inflammatory reaction and a mass of connective tissue were observed around the interface of bones and implants. Implants broke up with bones, the gap was obvious and the osteointegration was bad. Conclusions：New bone formation was inhibited in peri-implant area in tibias of rats, which caused inadequacy of bone-implant osteointegration．

Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｅｖａｌｕａｔｅ ｔｈｅ ｅｆｆｅｃｔ ｏｆ ｍｉｃｒｏｗａｖｅ ａｎｄ ｗａｔｅｒｂａｔｈ ｐｏｓｔｐｏｌｙｍｅｒｉｚａｔｉｏｎ ｔｒｅａｔｍｅｎｔｓ ｏｎ ｔｈｅ ｃｙｔｏｔｏｘｉｃｉｔｙ ｏｆ ｔｗｏｋｉｎｄｓ ｏｆ ｐｒｏｖｉｓｉｏｎａｌ ｒｅｓｔｏｒａｔｉｏｎ ｍａｔｅｒｉａｌｓ，ｐｏｌｙｍｅｔｈｙｌ ｍｅｔｈａｃｒｙｌａｔｅ ａｎｄ ｂｉｓａｃｒｙｌ ｃｏｍｐｏｓｉｔｅ ｒｅｓｉｎ． Ｍｅｔｈｏｄｓ：１８ ｃｙｌｉｎｄｅｒｓ （１０ ｍｍ ｉｎ ｄｉａｍｅｔｅｒ ａｎｄ ２ ｍｍ ｉｎ ｔｈｉｃｋｎｅｓｓ）ｆｏｒ ａｓｓｅｓｓｉｎｇ ｃｙｔｏｔｏｘｉｃｉｔｙ ｗｅｒｅ ｐｒｅｐａｒｅｄ ｉｎ ｅａｃｈ ｍａｔｅｒｉａｌ ａｎｄ ｄｉｖｉｄｅｄ ｉｎｔｏ ｔｈｒｅｅ ｇｒｏｕｐｓ，ｗｉｔｈ ６ ｓｐｅｃｉｍｅｎｓ ｉｎ ｅａｃｈ． Ｔｈｅ ｔｈｒｅｅ ｇｒｏｕｐｓ ｗｅｒｅ ｍｉｃｒｏｗａｖａ ｇｒｏｕｐ，ｗａｔｅｒｂａｔｈ ｇｒｏｕｐ，ａｎｄ ｃｏｎｔｒｏｌ ｇｒｏｕｐ． Ｓｐｅｃｉｍｅｎｓ ｉｎ ｔｈｅ ｃｏｎｔｒｏｌ ｇｒｏｕｐ ｗｅｒｅ ｌｅｆｔｕｎｔｒｅａｔｅｄ． Ｓｐｅｃｉｍｅｎｓ ｉｎ ｍｉｃｒｏｗａｖｅ ｇｒｏｕｐ ｗｅｒｅ ｔｒｅａｔｅｄ ｗｉｔｈ ｍｉｃｒｏｗａｖｅ ｉｒｒａｄｉａｔｉｏｎ ｆｏｒ ３ ｍｉｎ ａｔ ５００ Ｗ． Ｉｎ ｗａｔｅｒｂａｔｈ ｇｒｏｕｐ，ｓｐｅｃｉｍｅｎｓｗｅｒｅ ｓｕｂｍｉｔｔｅｄ ｔｏ ｉｍｍｅｒｓｉｏｎ ｉｎ ｗａｔｅｒ ａｔ ５５°Ｃ ｆｏｒ ３０ ｍｉｎ． Ｌｅａｃｈｉｎｇ ｌｉｑｕｏｒ ｏｆ ｔｈｅ ｓｐｅｃｉｍｅｎｓ ｗａｓ ｕｓｅｄ ｔｏ ａｓｓｅｓｓ ｔｈｅ ｃｙｔｏｔｏｘｉｃｉｔｙ ｏｆ ｃｅｌｌｓｂｙ ｍｅａｎｓ ｏｆ ｍｅｔｈｙｌｔｅｔｒａｚｏｌｉｕｍ ｔｅｓｔ． Ｒｅｓｕｌｔｓ：Ｆｏｒ ｐｏｌｙｍｅｔｈｙｌ ｍｅｔｈａｃｒｙｌａｔｅ ｍａｔｅｒｉａｌ，ｔｈｅ ｍｅａｎ ｐｅｒｃｅｎｔａｇｅ ｖｉａｂｉｌｉｔｙ ｏｆ ｃｏｎｔｒｏｌ ｇｒｏｕｐ （４７．６ ±３． ６）％ ｗａｓ ｓｉｇｎｉｆｉ ｃａｎｔｌｙ ｌｏｗｅｒ ｔｈａｎ ｔｈｏｓｅ ｏｆ ｍｉｃｒｏｗａｖｅ ｇｒｏｕｐ （７８． ４ ±４． ３）％ ａｎｄ ｗａｔｅｒ ｂａｔｈ ｇｒｏｕｐ （５４． ４ ±４． ３）％ ． Ｔｈｅｒｅ ｗａｓｓｉｇｎｉｆｉｃａｎｔ ｄｉｆｆｅｒｅｎｃｅ ｂｅｔｗｅｅｎ ｅａｃｈ ｐａｉｒ ｏｆ ｔｈｅ ｔｈｒｅｅ ｇｒｏｕｐｓ（Ｐ ＜０． ０５）． Ｆｏｒ ｂｉｓａｃｒｙｌ ｃｏｍｐｏｓｉｔｅ ｒｅｓｉｎ，ｔｈｅｒｅ ｗａｓ ｎｏ ｓｔａｔｉｓｔｃａｌｌｙ ｄｉｆｆｅｒｅｎｃｅ ｉｎ ｔｈｅ ｍｅａｎ ｐｅｒｃｅｎｔａｇｅ ｏｆ ｖｉａｂｉｌｉｔｙ ａｍｏｎｇ ｔｈｅ ｔｈｒｅｅ ｇｒｏｕｐｓ（Ｐ ＞０． ０５），ａｎｄ ｃｙｔｏｔｏｘｉｃ ｗａｓ ｉｎ ｇｒａｄｅ Ｉ． Ｃｏｎｃｌｕｓｉｏｎｓ：Ｍｉｃｒｏｗａｖｅ ａｎｄｗａｔｅｒｂａｔｈ ｐｏｓｔｐｏｌｙｍｅｒｉｚａｔｉｏｎ ｔｒｅａｔｍｅｎｔｓ ｃａｎ ｒｅｄｕｃｅ ｔｈｅ ｃｙｔｏｔｏｘｉｃｉｔｙ ｏｆ ｐｏｌｙｍｅｔｈｙｌ ｍｅｔｈａｃｒｙｌａｔｅ ｍａｔｅｒｉａｌ ｓｉｇｎｉｆｉｃａｎｔｌｙ，ｗｈｉｌｅ ｈａｖｅ ｌｉｔｔｌｅｅｆｆｅｃｔ ｏｎ ｔｈｅ ｂｉｓａｃｒｙｌ ｃｏｍｐｏｓｉｔｅ ｒｅｓｉｎ．

Abstract: Objective To evaluate the wear resistant of heat-cured base resin to three mechanical denture cleaning. Methods 64 samples were made, 60 of which were divided into 4 groups. Then, 3 groups were cleaned with three mechanical cleaning methodes separately. After cleaning, each group were divided into 3 subgroups to immerse into orange juice , dentilave or medicinal wine for 4 weeks. Baseline color was measured using a colorimeter. Further color measurements were made after 4 weeks of immersion. Compared ΔE with the results using a one-way ANOVA. The other 4 samples were made to observe by Electron microscopic. Results The mechanical cleaning have no effects on acceleration resin’s staining (P﹥0.05) except cleaning by tooth paste; cleaning by tooth paste showed wear on base resin, while cleaning with water and dental paste without wear effect. Conclusions Dentipur showed no wear on the heat-cured base resin, and did not acceleration resin’s staining. But thooth paste did.

[Abstract] Objective：Study of effects of rotary and reciprocating scrubbing methods on three cosmetic repair materials: E-max heat-pressed ceramic, Ceramage polymer-ceramic, nano-resins. Methods：12 specimens were made out of E-max heat-pressed ceramic, Ceramage polymer-ceramic, and nano-resin seperately. The specimens were divided into two subgroups according to different scrubbing methods. At room temperature, use self-made electric toothbrush brushing device，and a tube of tooth paste as wear medium to scrub the specimens in three kinds of materials. Each specimen was brushed for 194.6min, and the brushing force was 200 N. Weight loss, roughness and color change of three kinds of specimens were calculated before and after scrub. The results were analyzed by one-way ANOVA. Results：With the two scrubbing methods, the resin group and the polymer ceramic group both had weight loss (P <0.05). The weight loss of the resin group was more than that of the polymer ceramic group. In the nano resin group, weight loss of rotating scrub subgroup was more than that of reciprocating brush subgroup. As for changes in roughness value, rotary scrub polymer ceramic group and resin group had no significant changes. The E-max group had increase in roughness value(P <0.05). Besides, roughness value of the three groups of materials showed an increasing trend with reciprocating scrub method, among which the heat-pressed ceramic group increased most and there was no significant difference in the roughness changes between resin and polymer ceramic materials. The resin group had the biggest color changes before and after the experiment (P <0.05), Ceramage polymer ceramic came the second, and E-max group had no significant color change. There was significant difference of color changes between the resin group and E-max group(P<0.05) Conclusions：Electric brushing can cause weight loss and color changes of resin and polymer ceramic, cause increase in surface roughness values of heat-pressed ceramic. Rotary brush causes greater wear of the resin than reciprocating scrub, and reciprocating scrub causes increase in roughness values of the three kinds of materials. The two methods make no difference in color change of materials.

[Abstract] In the process of designing superscript-subscript tooth notation system, some aspects of this system such as the name of this system, the concepts of tooth identification and tooth identification character, and how to name permanent teeth and deciduous teeth are analyzed. Solutions for these problems in this system are proposed.

Oral mucositis is a common side effect of cancer therapies, particularly radiation therapy for head and neck cancer and various forms of chemotherapy. Radiation-induced oral mucositis has got a higher incidence and much severer than the one caused by chemotherapy. In addition to pain, dysphagia, secondary infection and dystrophia, severe oral mucositis limits cancer treatment. This article summarized the existing international and national situation of animal model for oral mucositis and its ways of prevention.