关键词: 衰老细胞, 羧基荧光素二醋酸盐琥珀酰亚胺酯, 衰老相关β-半乳糖苷酶

Abstract: Objective:?To explore the feasibility of using CFSE (carboxyfluorescein succinimidyl amino ester, CFSE) to sort senescent cells. Methods:?Senescent cells were induced by the CDK4/6 inhibitor LY2835219 on Cal27. CFSE staining was performed immediately after withdrawal of LY2835219, and then cells were cultured in the dark. The cells were stained with SA-β-gal on days 0, 2, 4, 6 and 8 after withdrawal. The cells with high fluorescence intensity of CFSE were sorted by a flow cytometer at a wavelength of 488 nm. Results:?Cells labeled with CFSE were consistent with SA-β-gal staining. The rate of positive result of SA-β-gal in the CFSE-high group sorted by CFSE on the 4th and 6th days after withdrawal was significantly higher than that in the unsorted group (P＜0.05). Conclusions:?Using CFSE to sort senescent cells is feasible, which could obtain live senescent cells.

Key words: senescence cell, carboxyfluorescein succinimidyl amino este, SA-β-gal