关键词: 卵泡抑素样蛋白1, 骨髓间充质干细胞, 成骨分化, 炎症

Abstract: Objective：To investigate whether follistatin-like protein 1 (FSTL1) can affect the osteogenic performance of mouse bone marrow mesenchymal stem cells (mBMSCs) in an inflammatory environment.?Methods:?mBMSCs were transfected with lentivirus to change the expression of FSTL1, and the proliferation activity of these cells was detected by CCK-8experiment. The mBMSCs transfected with lentivirus were stimulated with 10?ng/mL TNF-α, and then induced into osteoblasts.?Alkaline phosphatase (ALP) and alizarin red staining experiments were used to detect the changes of osteogenic ability of mBMSCs; ?the expression of collagen type Ⅰ collagen (Col1), osteopontin (Opn), osteocalcin (Ocn) was detected by real-time quantitative PCR.?Results:?Lentivirus transfection could effectively change the expression of FSTL1 gene in mBMSCs, and there was no significant difference in the proliferation activity of these cells (P＞0.05). In the inflammatory environment containing 10?ng/mL TNF-α, compared with the control group, mBMSCs overexpressing FSTL1 had lighter ALP and alizarin red staining results after osteogenic induction, and the expression of Col1, Opn, and Opn genes decreased (P＜0.05)；mBMSCs knockeddown FSTL1 had darker ALP and alizarin red staining results after osteogenic induction, and the expression of Col1, Opn, and Opn genes increased (P＜0.05). ?Conclusions:?FSTL1 promotes the inhibitory effect of inflammation on the osteogenic performance of mBMSCs, and inhibiting FSTL1 can effectively reduce the effect of inflammation on BMSCs.

Key words: follistatin-like protein 1, bone marrow mesenchymal stem cells, Inflammation