›› 2016, Vol. 7 ›› Issue (1): 54-56.

• 综述 • 上一篇    

大鼠牙周膜干细胞的分离、培养及应用的研究进展

刘努1,隋秉东2,赵盼2,刘琪1   

  1. 1. 遵义医学院附属口腔医院
    2. 第四军医大学口腔医院组织工程研发中心
  • 收稿日期:2015-07-30 修回日期:2016-01-19 出版日期:2016-03-25 发布日期:2016-04-06
  • 通讯作者: 刘努 E-mail:liunu1987@126.com
  • 基金资助:
    国家自然科学基金青年科学基金项目

Progress in the isolation and culture of rat periodontal ligament stem cells

  • Received:2015-07-30 Revised:2016-01-19 Online:2016-03-25 Published:2016-04-06

摘要: 大鼠牙周膜干细胞的分离、培养方法与人牙周膜干细胞有着不同之处,分离、培养大鼠牙周膜干细胞存在磨牙小、牙周膜组织来源少以及拔牙困难等难点。然而,在基础研究中,大鼠牙周炎模型的建立应用广泛,分离、培养大鼠牙周膜干细胞用以探讨牙周炎致病机制,明确机体内环境改变是否通过影响牙周膜干细胞的生物学性能导致牙周组织破坏严重与修复困难具有重要作用;另一方面,大鼠牙周膜干细胞分离、培养方法建立后,可以尝试从干细胞水平为治疗牙周病与促进牙周组织再生提供可靠的理论依据。因此,综述分离、培养大鼠牙周膜干细胞的可行方法有着十分重要的意义,有十分可观的应用前景。

Abstract: Significant methodological differences exist in the isolation and culture ofPDLSCs(PDLSCs) from rat and human. The isolation and culture of rat PDLSCs is restricted by theobstructions ofthe small donor molars, relativelimitedperiodontal ligament sources, and the difficulty of tooth extraction. However, the establishment of rat model of periodontitis is widely usedin the basic research, the isolation and culture of rat PDLSCs are of vital necessity for exploring the pathology of periodontitis. Furthermore, they are of additional importance for finding out whether the environmental changesimpair biological properties of PDLSCsto cause serious damage of periodontal tissues and the difficulties in regeneration.On the other hand, after the establishment of the methodology in the isolation and culture of rat PDLSCs, theoretical basis of stem cell-based periodontitis treatment and periodontal regeneration could be provided. Therefore, it is vitally significant to summarize the isolation and culture of rat PDLSCs that have promising applicable prospects.