多巴胺与Ⅰ型胶原对MC3T3-E1细胞初期粘附形态的影响

›› 2018, Vol. 9 ›› Issue (4): 187-190.

多巴胺与Ⅰ型胶原对MC3T3-E1细胞初期粘附形态的影响

李娜¹,章非敏²

1. 南京医科大学附属口腔医院
2. 南京医科大学口腔医学研究所附属口腔医院修复科

收稿日期:2018-11-05 修回日期:2018-11-29 出版日期:2018-12-25 发布日期:2019-01-07
通讯作者: 章非敏 E-mail:fmzhang@njmu.edu.cn
基金资助:
南京医科大学科技发展基金

Effects of dopamine and collagen on cell adhesion of MC3T3-E1 cells

Li-Na ¹,

Received:2018-11-05 Revised:2018-11-29 Online:2018-12-25 Published:2019-01-07

摘要/Abstract

摘要： 目的：探究多巴胺与多巴胺复合Ⅰ型胶原对成骨细胞MC3T3-E1初期粘附的影响。方法：分别在多巴胺改性、多巴胺复合Ⅰ型胶原改性的玻片和空白玻片上培养MC3T3-E1细胞。采用CCK-8法检测细胞体外增殖能力。通过场发射扫描电镜、激光共聚焦显微镜以及体式显微镜分别观察MC3T3-E1细胞早期粘附形态。结果：MC3T3-E1细胞在三组玻片上培养1、3、7 d的增殖结果差异无统计学意义（P>0.05）。相对于空白玻片组，MC3T3-E1细胞在多巴胺以及多巴胺复合Ⅰ型胶原组上铺展、粘附形态更好。结论：多巴胺促进成骨细胞粘附，多巴胺复合Ⅰ型胶原同样是理想的促粘附手段。两者在骨组织工程中是良好的表面改性手段。

Abstract: Objective： To investigate the effect of dopamine and dopamine/Type I collagen on the adhesion behavior of MC3T3-E1 cells. Methods： To evaluate the cell adhsion, the surface dopamine-coated (DOPA) and dopamine/collagen surface-coated (DOPA+Col) were prepared respectively. The surface without coating was set as control. MC3T3-E1 cells were chosen to seed onto the three groups (DOPA, DOPA+Col, Control), respectively. CCK-8 were used to evaluate the cell proliferation. The adhesion of MC3T3-E1 cells were observed by field emission scanning electron microscope (FESEM), confocal laser scanning microscope (CLSM) and stereo microscope. Results： There was no difference in cell proliferation at 1 d, 3 d or 7 d (P > 0.05) in each group. For adhesion morphology, MC3T3-E1 extended and adhered more on the DOPA and DOPA+Col treated surfaces. Conclusions： It revealed that dopamine or dopamine + Type I collagen were favorable for cell adhesion. Both treatments have good application prospects in bone tissue engineering.

李娜章非敏. 多巴胺与Ⅰ型胶原对MC3T3-E1细胞初期粘附形态的影响[J]. , 2018, 9(4): 187-190.

Li-Na. Effects of dopamine and collagen on cell adhesion of MC3T3-E1 cells[J]. , 2018, 9(4): 187-190.

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