聚己内酯/Ⅰ型胶原/氟磷灰石复合支架的制备及生物相容性研究

›› 2019, Vol. 10 ›› Issue (1): 12-16.

聚己内酯/Ⅰ型胶原/氟磷灰石复合支架的制备及生物相容性研究

范瑞瑞¹,李欣聪²,刘玉³,孙卫斌³,郭婷¹

1. 南京大学医学院附属口腔医院，南京市口腔医院
2. 南京大学医学院附属口腔医院
3. 南京大学口腔医学院

收稿日期:2019-01-10 修回日期:2019-02-25 出版日期:2019-03-25 发布日期:2019-04-02
通讯作者: 孙卫斌 E-mail:wbsun@nju.edu.cn
基金资助:
基于虚拟牙槽骨增量设计的三维打印多相复合支架个性化;氟磷灰石晶体-聚己内酯纳米纤维抗菌支架在诱导牙周组织再生中的研究;自噬在牙周组织再生中作用机理的研究

Preparation and biocompatibility of polycaprolactone/type I collagen/fluoroapatite composite scaffold

Received:2019-01-10 Revised:2019-02-25 Online:2019-03-25 Published:2019-04-02

摘要/Abstract

摘要： [摘要]目的：制备聚己内酯/Ⅰ型胶原/氟磷灰石复合支架用于牙周组织再生，并进行生物相容性及成骨诱导性能的检测。方法：制备聚己内酯/Ⅰ型胶原静电纺丝纳米纤维膜，并在其表面合成氟磷灰石矿化涂层，通过扫描电镜观察其形貌。同时分离培养人牙周膜细胞并接种于支架表面，通过CCK-8法检测细胞增殖情况，通过碱性磷酸酶染色、茜素红染色和Von Kossa染色观察复合支架对人牙周膜细胞成骨分化的影响。结果：聚己内酯/Ⅰ型胶原/氟磷灰石复合支架呈三维网状结构，氟磷灰石晶体分布于支架表面，CCK-8结果证明人牙周膜细胞在复合支架上增殖情况良好。碱性磷酸酶染色、茜素红染色和Von Kossa染色结果提示复合支架可可诱导人牙周膜细胞成骨分化。结论：聚己内酯/Ⅰ型胶原/氟磷灰石复合支架生物相容性良好并具有成骨诱导潜能，有望作为牙周组织工程支架材料。

Abstract: [Abstract]Objective: Polycaprolactone/type I collagen/fluoroapatite composite scaffolds were prepared for periodontal tissue regeneration and biocompatibility and osteogenic induction were tested. Methods: Polycaprolactone/type I collagen electrospun nanofiber membrane was prepared, and the fluoroapatite mineralized coating was synthesized on the surface. The morphology was observed by scanning electron microscopy. At the same time, human periodontal ligament cells were isolated and cultured on the surface of the scaffold, and the cell proliferation was detected by CCK-8 method. The effects of composite scaffold on osteogenic differentiation of human periodontal ligament cells were observed by alkaline phosphatase staining, alizarin red staining and Von Kossa staining.Results: The polycaprolactone/type I collagen/fluoroapatite composite scaffold showed a three-dimensional network structure, and the fluoroapatite crystals were distributed on the surface of the scaffold. The CCK-8 results showed that the human periodontal ligament cells proliferated well on the composite scaffold. Alkaline phosphatase staining, alizarin red staining and Von Kossa staining results suggest that the composite scaffold can induce osteogenic differentiation of human periodontal ligament cells. Conclusion: The polycaprolactone/type I collagen/fluoroapatite composite scaffold has good biocompatibility and osteogenic induction potential, and it is expected to be used as a scaffold material for periodontal tissue engineering.

中图分类号:

R781.4

范瑞瑞李欣聪刘玉孙卫斌郭婷. 聚己内酯/Ⅰ型胶原/氟磷灰石复合支架的制备及生物相容性研究[J]. , 2019, 10(1): 12-16.

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