臭氧化油对牙龈卟琳单胞菌的体外抑菌作用

›› 2019, Vol. 10 ›› Issue (1): 22-25.

臭氧化油对牙龈卟琳单胞菌的体外抑菌作用

葛舒¹,李璐²,吴程宇¹,周逸¹,徐艳³

1. 南京医科大学口腔疾病研究江苏省重点实验室
2. 南京医科大学附属口腔医院牙周科
3. 南京医科大学口腔医学院

收稿日期:2019-02-19 修回日期:2019-03-01 出版日期:2019-03-25 发布日期:2019-04-02
通讯作者: 徐艳 E-mail:xuyan@njmu.edu.cn
基金资助:
江苏高校优势学科建设工程资助项目

Antibacterial activity of ozonated oil against Porphyromonas gingivalis in vitro

Received:2019-02-19 Revised:2019-03-01 Online:2019-03-25 Published:2019-04-02

摘要/Abstract

摘要： 摘要：目的：评估臭氧化油对牙龈卟琳单胞菌的体外抑菌作用。方法：通过琼脂扩散法评估臭氧化油对牙龈卟琳单胞菌的抑制作用；采用肉汤微量稀释法检测臭氧化油对牙龈卟琳单胞菌的最小抑菌浓度（MIC）及最小杀菌浓度（MBC）；利用激光共聚焦显微镜(confocal laser scanning microscopy, CLSM)和扫描电镜(scanning electron microscopy, SEM)观察臭氧化油处理后对细菌活力、生物膜结构以及细胞形态的影响。结果：臭氧化油明显抑制了牙龈卟琳单胞菌的生长，抑菌圈直径为68.13 mm，差异具有统计学意义（P < 0.05）；臭氧化油对牙龈卟琳单胞菌的MIC和 MBC均为0.04 mg/mL；CLSM和SEM图像证实了臭氧化油处理牙龈卟琳单胞菌后未见生物膜结构的形成，细菌活力明显降低，细菌总量显著减少，细胞完整的形态被破坏，细胞皱缩和裂解。结论：臭氧化油对牙龈卟琳单胞菌及其生物膜的形成均有较强的抑制作用。

Abstract: Abstract: Objective: To evaluate the antibacterial activity of ozonated oil on Porphyromonas gingivalis (P. gingivalis). Methods: Agar diffusion assay was used to investigate the sensitivity of P. gingivalis to ozonated oil. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth microdilution method. The effects of ozonated oil on bacterial viability, biofilm structure and cell morphology were observed by Laser confocal microscopy (CLSM) and scanning electron microscopy (SEM). Results: Ozonated oil significantly inhibited the growth of P. gingivalis with the inhibition diameter of 68.13 mm. And the MIC and MBC of ozonated oil on P. gingivalis were 0.04 mg/mL. CLSM and SEM images confirmed that after ozonated oil treatment, no dense biofilm structure was observed, the viability and density of biofilm decreased, the intact cell shape was damaged and the cells shrink and lysed. Conclusions: Ozonated oil had a strong inhibitory effect on planktonic and biofilm forms of P. gingivalis.

葛舒李璐吴程宇周逸徐艳. 臭氧化油对牙龈卟琳单胞菌的体外抑菌作用[J]. , 2019, 10(1): 22-25.

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