钟状期小鼠上颌第一磨牙的拔除与原位移植模型

doi:10.3969/j.issn.1674-8603.2020.02.003

›› 2020, Vol. 11 ›› Issue (2): 76-80.doi: 10.3969/j.issn.1674-8603.2020.02.003

钟状期小鼠上颌第一磨牙的拔除与原位移植模型

李星瀚¹,²,田卫东²

1. 深圳大学总医院
2. 四川大学华西口腔医学院

收稿日期:2019-11-04 修回日期:2020-04-25 出版日期:2020-06-25 发布日期:2020-07-08
通讯作者: 田卫东 E-mail:drtwd@sina.com
基金资助:
国家重点研发计划;深圳大学总医院人才助推计划

Extraction and orthotopic transplantation model of the upper first molar at bell stage in mice

Xing-Han LI¹,²,Wei-Dong TIAN

Received:2019-11-04 Revised:2020-04-25 Online:2020-06-25 Published:2020-07-08
Contact: Wei-Dong TIAN E-mail:drtwd@sina.com

摘要/Abstract

摘要： 目的：为研究牙再生、牙根发育和牙萌出建立能模拟天然牙发育环境的动物模型。方法：选取钟状期小鼠上颌第一磨牙作为牙拔除与牙移植的研究对象，进行动物建模，并通过术后影像学和组织学观察与肾被膜下移植模型进行对比。结果：出生后第8天的小鼠上颌第一磨牙牙胚处于牙冠基本形成而牙根即将开始发育的阶段，适合用于本模型。相较肾被膜下移植模型，本模型移植牙胚的牙根发育更接近天然牙发育。结论：钟状期小鼠上颌第一磨牙牙胚拔除及移植模型是观察牙根发育和牙萌出的良好选择。

关键词: 牙胚, 动物模型, 牙再生, 牙发育

Abstract: Objective:?Establishing an animal model which can simulate the natural tooth developing environment in physiological condition for the researches of tooth regeneration, root development and tooth eruption. Methods:?The upper first molar of mouse at bell stage is used in this model for extraction and transplantation, comparing with the subrenal capsule transplantation model by radiological and histological observation postoperatively. Results:?The tooth germ of upper first molar by the postnatal 8th day has almost formed the crown and the root development is about to begin, which is a suitable subject of study in this model. Comparing with that in the subrenal capsule transplantation model, the root development of transplanted tooth germ in this model shows more similar to that of the natural tooth. Conclusions:?The extraction and orthotopic transplantation model of the upper first molar at bell stage in mice is a desired option for the observation of root development and tooth eruption.

Key words: Tooth germ, animal model, tooth regeneration, Tooth development

李星瀚田卫东. 钟状期小鼠上颌第一磨牙的拔除与原位移植模型[J]. 口腔生物医学, 2020, 11(2): 76-80.

Xing-Han LI Wei-Dong TIAN. Extraction and orthotopic transplantation model of the upper first molar at bell stage in mice[J]. Oral Biomedicine, 2020, 11(2): 76-80.

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钟状期小鼠上颌第一磨牙的拔除与原位移植模型

Extraction and orthotopic transplantation model of the upper first molar at bell stage in mice

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