外周血miR-299-5p和miR-127-3p的表达水平与非综合征型唇腭裂相关性研究

口腔生物医学 ›› 2021, Vol. 12 ›› Issue (1): 30-33.

外周血miR-299-5p和miR-127-3p的表达水平与非综合征型唇腭裂相关性研究

张兆华¹，高雨蔚¹，宋红权¹，隗子文¹，杨帆²，焦晓辉¹

1. 哈尔滨医科大学附属第一医院口腔颌面外科，哈尔滨医科大学口腔医学院
2. 黑龙江中医药大学附属第一医院口腔科

收稿日期:2020-12-01 修回日期:2021-03-03 出版日期:2021-03-25 发布日期:2021-04-01
通讯作者: 焦晓辉 E-mail:1814215686@qq.com
基金资助:
国家重点研发计划（2016YFC100504）

Correlation between expression level of miR-299-5p and miR-127-3p in peripheral blood and nonsyndromic orofacial clefts

1. Department of Oral Maxillofacial Surgery, The First Affiliated Hospital of Harbin Medical University,School of Stomatology, Harbin Medical University
2. Department of Oral Maxillofacial Surgery, The First Affiliated Hospital of Harbin Medical University,School of Stomatology, Harbin Medical University

Received:2020-12-01 Revised:2021-03-03 Online:2021-03-25 Published:2021-04-01

摘要/Abstract

摘要： 目的：研究外周血中miR-299-5p和miR-127-3p的表达水平与非综合征性唇腭裂（NSOC）的相关性。方法：选取于哈尔滨医科大学附属第一医院就诊的唇裂伴或不伴腭裂（CL/P）患儿、单纯腭裂（CPO）患儿和健康儿童各10例，采用实时荧光定量RT-PCR（qRT-PCR）检测各组外周血中miR-299-5p和miR-127-3p的表达水平，采用受试者工作特征（ROC）曲线和曲线下面积（AUC）分析其对NSOC的诊断效能。结果：与健康对照组相比，CL/P组和CPO组miR-299-5p和miR-127-3p的表达下调，差异有统计学意义（P＜0.05）。miR-299-5p、miR-127-3p及其联合诊断NSOC的AUC分别为0.770（95%?CI：0.558~0.982）、0.810（95%?CI：0.651~0.969）、0.915（95%?CI：0.754~0.985）。结论：NSOC患儿外周血miR-299-5p和miR-127-3p表达下调，可能与NSOC的发生有关，其可能成为诊断NSOC的潜在生物标记物，且二者联合诊断效能更优。

关键词: 非综合征性唇腭裂, miR-299-5p, miR-127-3p, 生物标记物

Abstract: Objective:The aim was to investigate correlation between expression level of miR-299-5p and miR-127-3p in peripheral blood and nonsyndromic orofacial clefts (NSOC). Methods:Ten cleft lip with or without cleft palate (CL/P) patients, 10 cleft palate only (CPO) and 10 healthy children were selected from the First Affiliated Hospital of Harbin Medical University, using real-time RT-PCR（qRT-PCR）to detect the relative expression of miR-299-5p miR-127-3p in the peripheral blood from three groups. The receiver operating characteristic (ROC) curve and area under the curve (AUC) were applied to analyze the diagnostic efficacy of miR-299-5p and miR-127-3p for NSOC. Results:Compared with the healthy control group, the expression of miR-299-5p and miR-127-3p in CL/P group and CPO group was down-regulated, and the difference was statistically significant (P<0.05). The AUC of miR-299-5p and miR-127-3p in the diagnosis of NSOC were 0.770 and 0.810, respectively. The AUC of miR-299-5p and miR-127-3p in the combined diagnosis of NSOC was 0.915. Conclusions:The down-regulation of miR-299-5p and miR-127-3p in peripheral blood of children with NSOC may be related to the progression of NSOC, which may become a potential biomarker for the diagnosis of NSOC, and the combination of the two miRNAs is more effective.

Key words: nonsyndromic orofacial clefts, ?miR-299-5p, ?miR-127-3p, ?biomarker

张兆华高雨蔚宋红权隗子文杨帆焦晓辉. 外周血miR-299-5p和miR-127-3p的表达水平与非综合征型唇腭裂相关性研究[J]. 口腔生物医学, 2021, 12(1): 30-33.

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