miR-206-3p调控RAW264.7细胞向破骨细胞分化的体外研究

口腔生物医学 ›› 2021, Vol. 12 ›› Issue (2): 87-90.

miR-206-3p调控RAW264.7细胞向破骨细胞分化的体外研究

吴青蘅¹,王徐雯¹,朱昕妍¹,山轶凡¹,周宣¹,顾嘉雯²,郭舒瑜²

1. 南京医科大学口腔医学院
2. 南京医科大学口腔疾病研究江苏省重点实验室，南京医科大学附属口腔医院正畸科

收稿日期:2020-11-25 修回日期:2021-03-11 出版日期:2021-06-25 发布日期:2021-06-25
通讯作者: 郭舒瑜 E-mail:syguo@njmu.edu.cn
基金资助:
国家自然科学基金;江苏省自然科学基金;江苏省高等学校自然科学研究项目

Effects of miR-206-3p on the differentiation of RAW264.7 cells into osteoclasts by regulating NFATc1

Received:2020-11-25 Revised:2021-03-11 Online:2021-06-25 Published:2021-06-25
Contact: Shu-yu GUO E-mail:syguo@njmu.edu.cn

摘要/Abstract

摘要： 目的：探究miR-206-3p对破骨细胞生成的影响。方法：RAW264.7细胞体外诱导破骨分化，过表达或抑制其miR-206-3p，实时定量RT-PCR检测miR-206-3p表达水平。通过纤维状肌动蛋白染色、抗酒石酸酸性磷酸酶染色观察破骨细胞形成，实时定量RT-PCR及Western Blot检测破骨细胞分化相关基因——活化T细胞核因子1（NFATc1）的表达。结果：过表达miR-206-3p后，破骨细胞生成受到抑制，NFATc1表达水平降低，而敲减miR-206-3p后可促进破骨细胞的生成，NFATc1表达显著上调（P<0.05）。结论：miR-206-3p通过影响NFATc1的表达，调控RAW264.7细胞向破骨细胞分化。

Abstract: Objective:?To investigate the effect of miR-206-3p on osteoclastogenesis. Methods:?RAW264.7 cells were induced into osteoclasts in vitro. The knockdown and over expression efficiencies of miR-206-3p were examined by real-time fluorescence quantitative PCR (qRT-PCR). Then F-actin staining and trap-resistant acid phosphatase (TRAP) staining were performed to detect the osteoclastogenesis, and the expression of osteoclast differentiation-related gene NFATc1 was evaluated by qRT-PCR and Western Blot. Results:?The overexpression of miR-206-3p significantly inhibited osteoclastogenesis and the expression of NFATc1, while the inhibition of miR-206-3p did the opposite effect (P<0.05). Conclusions:?miR-206-3p regulates the differentiation of RAW264.7 cells into osteoclasts by targeting the expression of NFATc1.

吴青蘅王徐雯朱昕妍山轶凡周宣顾嘉雯郭舒瑜. miR-206-3p调控RAW264.7细胞向破骨细胞分化的体外研究[J]. 口腔生物医学, 2021, 12(2): 87-90.

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