骨质疏松小鼠骨内糖胺聚糖的变化

口腔生物医学 ›› 2021, Vol. 12 ›› Issue (2): 91-94.

骨质疏松小鼠骨内糖胺聚糖的变化

牛萍萍¹,²,李俊辉³,薛徽¹,孙瑶⁴,周济²,张帅²

1. 齐齐哈尔医学院第一附属医院口腔科
2. 同济大学附属口腔医院
3. 同济大学口腔医学院
4. 同济大学口腔医学院?同济大学附属口腔医院种植科，上海牙组织修复与再生工程技术研究中心

收稿日期:2020-12-01 修回日期:2021-03-08 出版日期:2021-06-25 发布日期:2021-06-25
通讯作者: 孙瑶 E-mail:yaosun@tongji.edu.cn
基金资助:
国家自然基金

Changes of glycosaminoglycans in bone of osteoporosis mice

Received:2020-12-01 Revised:2021-03-08 Online:2021-06-25 Published:2021-06-25

摘要/Abstract

摘要： 目的：分析骨质疏松小鼠骨组织中糖胺聚糖（GAG）的含量变化。方法：构建雌激素缺失骨质疏松小鼠动物模型，通过Micro-CT和组织学切片染色确定模型构建是否成功；利用甲苯胺蓝染色和实时定量RT-PCR对比分析假手术组和骨质疏松组小鼠股骨GAG含量及相关合成酶基因表达差异。结果：手术摘除双侧卵巢三个月后，小鼠总脂肪增加、体重增加、子宫湿重减少、骨密度降低，建模成功。与假手术组相比，骨质疏松小鼠骨组织GAG含量减少，GAG合成酶基因表达水平下降，差异有统计学意义（P<0.05）。结论：GAG与骨质疏松发生发展密切相关。

关键词: 骨质疏松症, 糖胺聚糖, 小鼠

Abstract: Objective:?To analyze the changes of glycosaminoglycan (GAG) in bone tissue of osteoporosis mice. Methods:?We constructed the animal model of hormone-deficient osteoporosis mice, and assessed whether the model was established successfully by Micro CT and histological section staining. The content of femoral GAG analyzed by Toluidine blue staining and the expression of related gene measured by RT-qPCR were compared between the two groups. Results: Three months after surgery, the experimental mice had an increased total fat and body weight, while the weight of uterus and bone density are decreased. The content of GAG in bone tissue decreased and mRNA expression level of related genes to GAG chain decreased (P<0.05). Conclusion: GAG is closely related to the occurrence and development of osteoporosis.

Key words: osteoporosis, glycosaminoglycan, mice

牛萍萍李俊辉薛徽孙瑶周济张帅. 骨质疏松小鼠骨内糖胺聚糖的变化[J]. 口腔生物医学, 2021, 12(2): 91-94.

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