具核梭杆菌感染的巨噬细胞对人舌鳞癌细胞系Cal-27细胞增殖与迁移的影响

口腔生物医学 ›› 2021, Vol. 12 ›› Issue (3): 166-169.

具核梭杆菌感染的巨噬细胞对人舌鳞癌细胞系Cal-27细胞增殖与迁移的影响

方媛春¹,孙颖²,胡永³

1. 苏州科技城医院
2. 南京医科大学口腔医学院
3. 南京医科大学附属苏州科技城医院

收稿日期:2020-12-24 修回日期:2021-05-24 出版日期:2021-09-25 发布日期:2021-09-27
通讯作者: 孙颖 E-mail:ebolasun@163.com
基金资助:
南京医科大学科技发展基金

Effects of macrophages induced by Fusobacterium nucleatum on proliferation and migration of tongue squamous cell carcinoma Cal-27 cells

Yong HU²

Received:2020-12-24 Revised:2021-05-24 Online:2021-09-25 Published:2021-09-27

摘要/Abstract

摘要： 目的：观察具核梭杆菌（F. nucleatum）诱导的巨噬细胞对人舌鳞癌细胞系Cal-27细胞增殖、迁移的影响。方法：F. nucleatum以感染复数（MOI）100∶1，刺激THP-1细胞来源的巨噬细胞，24 h后收集细胞条件培养液，以THP-1细胞及THP-1细胞来源巨噬细胞的条件培养液为对照。将获得的各组条件培养液与Cal-27细胞共培养，CCK-8法检测各组细胞增殖水平的变化，划痕实验检测Cal-27细胞迁移能力的变化。ELISA检测各组条件培养液中肿瘤坏死因子α（TNF-α）及白细胞介素（IL）-6表达水平。结果：与2个对照组相比，F. nucleatum诱导的巨噬细胞培养液上清使Cal-27细胞增殖减少（P＜0.05），相对迁移率增高（P＜0.05）。F. nucleatum刺激巨噬细胞后，条件培养液中TNF-α和IL-6水平均较刺激前明显增加（P＜0.05）结论：F. nucleatum感染能促进巨噬细胞分泌TNF-α和IL-6，进而可能促进Cal-27细胞迁移，抑制其增殖。

关键词: 具核梭杆菌, THP-1细胞, Cal-27细胞, 迁移, 增殖

Abstract: Objective:To observe the effects of infection induced by Fusobacterium nucleatum (F. nucleatum) on the proliferation and infiltration of tongue squamous cell carcinoma cell line Cal-27. Methods:Supernatant from THP-1 cells differentiated macrophages stimulated with F. nucleatum (multiplicity of infection was 100:1) for 24 h was collected, and supernatants from unstimulated THP-1 cells differentiated macrophages and THP-1 cells were collected as controls. Cal-27 cells were co-cultured with the above-mentioned supernatants and Cal-27 cells proliferation was detected by CCK-8 method. Migration of Cal-27 cells were evaluated by scratch assay. Expressions of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 were detected by ELISA. Results: F. nucleatum-induced macrophages conditioned medium reduced the proliferation but promoted the migration rate of Cal-27 cells in comparison with two control groups (P<0.05). The levels of TNF-α and IL-6 in macrophages stimulated with F. nucleatum were significantly higher than those without any stimulations (P<0.05). Conclusions:Stimulation of macrophages with F. nucleatum promoted TNF-α and IL-6 production, which may promote the migration but inhibit the proliferation of Cal-27 cells.

Key words: Fusobacterium nucleatum, THP-1 cells, Cal-27 cells, migration, proliferation

方媛春孙颖胡永. 具核梭杆菌感染的巨噬细胞对人舌鳞癌细胞系Cal-27细胞增殖与迁移的影响[J]. 口腔生物医学, 2021, 12(3): 166-169.

Yong HU. Effects of macrophages induced by Fusobacterium nucleatum on proliferation and migration of tongue squamous cell carcinoma Cal-27 cells[J]. Oral Biomedicine, 2021, 12(3): 166-169.

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