炎症微环境对于牙周膜干细胞与脐带间充质干细胞再生功能的影响

doi:10.3969/j.issn.1674-8603.2020.02.002

›› 2020, Vol. 11 ›› Issue (2): 71-75.doi: 10.3969/j.issn.1674-8603.2020.02.002

炎症微环境对于牙周膜干细胞与脐带间充质干细胞再生功能的影响

苏晓霞¹,雷凤珍²,王芮²,林亭廷²,王菲²,廖立³

1. 四川大学华西口腔医院
2. 西安交通大学医学院附属口腔医院
3. 四川大学口腔医院

收稿日期:2019-11-04 修回日期:2020-03-11 出版日期:2020-06-25 发布日期:2020-07-08
通讯作者: 廖立 E-mail:lliao@scu.edu.cn

The Influence of inflammatory micro-environment on regenerative capacity of PDLSCs and UCMSCs

Received:2019-11-04 Revised:2020-03-11 Online:2020-06-25 Published:2020-07-08

摘要/Abstract

摘要： 目的：比较炎症微环境对于牙周膜干细胞（PDLSCs）与脐带间充质干细胞（UCMSCs）再生能力的影响。方法：通过肿瘤坏死因子-α（TNF-α）刺激模拟炎症微环境，利用增殖实验和增殖标志基因细胞周期蛋白E1（CCNE1）、细胞周期蛋白D1（CCND1）检测比较PDLSCs与UCMSCs的增殖能力，AnnexinV/PI染色检测细胞凋亡，茜素红染色及成骨分化相关基因Runt相关转录因子2（RUNX2）、骨钙素（OCN）的表达水平，检测细胞成骨分化能力。结果：TNF-α处理导致PDLSCs的增殖能力和成骨分化能力显著降低（P＜0.001），凋亡细胞比例有较大升高；而TNF-α处理后UCMSCs的增殖能力和成骨分化能力仅轻度降低（P＜0.001），凋亡细胞比例轻度升高。结论：与PDLSCs相比，UCMSCs对炎症微环境的抵抗能力更强。

关键词: 牙周膜干细胞, 人脐带间充质干细胞, 炎症, 肿瘤坏死因子-α, 分化, 增殖

Abstract: Objective:The study aims to compare effect of inflammatory microenvironment on the regenerative capacitary of periodontal ligament stem cells (PDLSCs) andumbilical cord mesenchymal stem cells (UCMSCs). Methods：TNF-α was applied to mimic inflammatory microenvironment. Cell proliferation assay and proliferation marker genes CCNE1, CCND1 analysis were used to compare the proliferation capacity. AnnexinV/PI staining was used to detect cellular apoptosis. Alizarin red staining and osteogenesis marker genes RUNX2, OCN analysis were used to compare the capacity of osteogenic differentiation. Results：Treatment of TNF-α resulted in an obvious decrease in the proliferation and osteogenic differentiation capacity of PDLSCs, while an increase in apoptosis of PDLSCs. In contract, treatment of TNF-α leaded to a mild decrease in the capacity of proliferation and osteogenic differentiation in UCMSCs, and a slight increase in cellular apoptosis. Conclusions：Comparing with PDLSCs, UCMSCs are more resistant to the influence of inflammatory microenvironment.

Key words: periodontal ligament stem cells, Human umbilical cord mesenchymal stem cells, Inflammation, TNF-α, differentiation, proliferation

苏晓霞雷凤珍王芮林亭廷王菲廖立. 炎症微环境对于牙周膜干细胞与脐带间充质干细胞再生功能的影响[J]. 口腔生物医学, 2020, 11(2): 71-75.

参考文献 13

[1]	Zhang H, Liu SY, Zhu B, Xu Q, Ding Y, Jin Y.Composite cell sheet for periodontal regeneration: crosstalk between different types of MSCs in cell sheet facilitates complex periodontal-like tissue regeneration. Stem cell research & therapy7, 168 (2016).
[2]	Chen FM, Jin Y.Periodontal Tissue Engineering and Regeneration: Current Approaches and Expanding Opportunities. Tissue Eng Part B-Re16, 219-255 (2010).
[3]	Chen FM, et al.Treatment of periodontal intrabony defects using autologous periodontal ligament stem cells: a randomized clinical trial. Stem cell research & therapy7, 33 (2016).
[4]	Shang FQ, et al.Human Umbilical Cord MSCs as New Cell Sources for Promoting Periodontal Regeneration in Inflammatory Periodontal Defect. Theranostics7, 4370-4382 (2017).
[5]	Huang GTJ, Gronthos S, Shi S.Mesenchymal Stem Cells Derived from Dental Tissues vs. Those from Other Sources: Their Biology and Role in Regenerative Medicine. Journal of dental research88, 792-806 (2009).
[6]	Yang N, Li Y, Wang G, Ding Y, Jin Y, Xu YQ.Tumor necrosis factor-alpha suppresses adipogenic and osteogenic differentiation of human periodontal ligament stem cell by inhibiting miR-21/Spry1 functional axis. Differentiation97, 33-43 (2017).
[7]	Yang H, et al.Comparison of mesenchymal stem cells derived from gingival tissue and periodontal ligament in different incubation conditions. Biomaterials34, 7033-7047 (2013).
[8]	Sui BD, Hu CH, Zheng CX, Jin Y.Microenvironmental Views on Mesenchymal Stem Cell Differentiation in Aging. Journal of dental research95, 1333-1340 (2016).
[9]	Liao L, et al.Redundant miR-3077-5p and miR-705 mediate the shift of mesenchymal stem cell lineage commitment to adipocyte in osteoporosis bone marrow. Cell Death Dis4, e600 (2013).
[10]	Liao L, et al.TNF-α Inhibits FoxO1 by Upregulating miR-705 to Aggravate Oxidative Damage in Bone Marrow-Derived Mesenchymal Stem Cells during Osteoporosis. Stem Cells34, 1054-1067 (2016).
[11]	Yang N, et al.Tumor necrosis factor suppresses the mesenchymal stem cell osteogenesis promoter miR-21 in estrogen deficiencyinduced osteoporosis. Journal of Bone and Mineral Research28, 559-573 (2013).
[12]	Shioda M, et al.TNFalpha promotes proliferation of human synovial MSCs while maintaining chondrogenic potential. PloS one12, e0177771 (2017).
[13]	Ghali O, Chauveau C, Hardouin P, Broux O, Devedjian JC.TNF-alpha' s effects on proliferation and apoptosis in human mesenchymal stem cells depend on RUNX2 expression. J Bone Miner Res25, 1616-1626 (2010).

[1]	潘新华李烿烿叶露露朱国培. 全反式维甲酸联合阿帕替尼抑制口腔鳞癌细胞增殖体外研究[J]. 口腔生物医学, 2022, 13(1): 15-19.
[2]	徐瑞王锐高雪峰郭雨婷吕学超. 黄精多糖对乳牙牙髓干细胞增殖及成骨向分化的影响[J]. 口腔生物医学, 2022, 13(1): 40-44.
[3]	方媛春孙颖胡永. 具核梭杆菌感染的巨噬细胞对人舌鳞癌细胞系Cal-27细胞增殖与迁移的影响[J]. 口腔生物医学, 2021, 12(3): 166-169.
[4]	雷心怡张旗. 自噬在延期牙再植术后炎症性牙根吸收中的作用研究[J]. 口腔生物医学, 2021, 12(3): 149-154.
[5]	吴青蘅王徐雯朱昕妍山轶凡周宣顾嘉雯郭舒瑜. miR-206-3p调控RAW264.7细胞向破骨细胞分化的体外研究[J]. 口腔生物医学, 2021, 12(2): 87-90.
[6]	王桂芳蒋欣泉. 掺镁微弧氧化钛表面促进大鼠BMSCs成骨分化的研究[J]. 口腔生物医学, 2021, 12(2): 76-81.
[7]	史凡黄鉴栎刘梅王丹章非敏. 碳点示踪并促进大鼠骨髓间充质干细胞成骨分化的初步研究[J]. 口腔生物医学, 2021, 12(1): 5-11.
[8]	谢艾伦周楠陈晓艳刘超肖晶. 持续激活Fgf8对颌面部不同来源成肌细胞的差异性作用[J]. , 2020, 11(4): 214-221.
[9]	徐晓旭洪蕾蕾袁礼婵王晓亮马俊青. 二氧化钛纳米管负载京尼平交联的壳聚糖水凝胶体外生物活性的研究[J]. , 2020, 11(3): 164-167.
[10]	杨乔林刘朋郑云飞李巍然. miR-21对牙周膜干细胞炎症状态及成骨分化的影响[J]. , 2020, 11(2): 86-91.
[11]	金齐尧毛广艳邓威王晨星李怀奇王东苗叶金海. 炎性环境下FSTL1对小鼠骨髓间充质干细胞成骨性能的影响[J]. , 2020, 11(2): 120-124.
[12]	贾思洵张思诚陈国庆田卫东. 基底硬度调节成骨细胞体外生长及分化[J]. , 2020, 11(1): 13-18.
[13]	陈朕卢金金赵雪涛申玉芹. 线粒体DNA与牙周炎相关性研究进展[J]. , 2019, 10(4): 214-217.
[14]	刘静文杨东梅. EGF家族及其受体对间充质干细胞功能影响的研究进展[J]. , 2019, 10(3): 149-153.
[15]	李晓钰王红谷秀格魏福兰. 年龄因素对牙周膜干细胞多向分化能力的影响[J]. , 2019, 10(2): 78-82.

炎症微环境对于牙周膜干细胞与脐带间充质干细胞再生功能的影响

The Influence of inflammatory micro-environment on regenerative capacity of PDLSCs and UCMSCs

可视化

摘要/Abstract

引用本文

使用本文

参考文献 13

相关文章 15

编辑推荐

Metrics

本文评价