口腔生物医学 ›› 2022, Vol. 13 ›› Issue (1): 30-34.

• 论著 • 上一篇    下一篇

牙周基础治疗对体内一氧化氮水平和血压的影响

曹羡梓,付子波,陈佳璐,孙颖   

  1. 南京医科大学口腔医学院
  • 收稿日期:2022-02-16 修回日期:2022-03-06 出版日期:2022-03-25 发布日期:2022-03-21
  • 通讯作者: 孙颖 E-mail:ebolasun@163.com
  • 基金资助:
    国家自然科学基金;江苏高校优势学科建设工程资助项目

Effects of Non-surgical Periodontal Treatment on Nitric Oxide and Blood Pressure

  • Received:2022-02-16 Revised:2022-03-06 Online:2022-03-25 Published:2022-03-21

摘要: 目的:探讨牙周基础治疗对一氧化氮(NO)代谢和血压的影响。方法:纳入45名血压正常的中、重度牙周炎患者,记录牙周基础治疗前及治疗后1月患者的菌斑指数、牙龈指数、探诊深度、临床附着丧失(CAL)及血压,采用Griess法测量治疗前后唾液及血浆NO氧化产物亚硝酸钠(NaNO2)水平,实时定量PCR技术检测治疗前后龈下菌斑中牙龈卟啉单胞菌(P. gingivalis)、中间普氏菌(P. intermedia)及总菌水平,ELISA法检测治疗前后龈沟液(GCF)和血清中的白介素(IL)-6水平,以及血清C-反应蛋白(CRP)水平,并对治疗前血压、牙周临床指标、细菌数量、NaNO2、CRP、IL-6水平间的关系进行相关性分析。结果:牙周基础治疗后1月,患者各牙周临床指标、血压、唾液及血浆NaNO2水平、龈下菌斑中的P. gingivalis、P. intermedia及总菌水平、血清及GCF中的IL-6及CRP水平均显著下降(P<0.05)。治疗前收缩压与GCF中IL-6水平、唾液NaNO2水平、CAL均存在中度相关性;舒张压与总菌水平、P. intermedia存在中度相关性(P<0.05)。结论:牙周基础治疗能降低唾液及血浆NO水平,同时改善全身炎症状态,为伴高血压的牙周炎患者的治疗提供参考。

Abstract: Objective: The aim of this study was to explore the short-term effects of non-surgical periodontal treatment (NSPT) on NO metabolism and blood pressure control. Methods: Forty-five normotensive patients with periodontitis (stage Ⅲ or Ⅳ and grade B or C) were recruited and received NSPT. Periodontal parameters, including plaque index, gingival index, probing depth, clinical attachment loss (CAL) and blood pressure were recorded before and one month after NSPT. Levels of NO in salivary and plasma were explored by Griess reaction. Quantities of Porphyromonas gingivalis (P. gingivalis), Prevotella intermedia (P. intermedia) and total bacteria, in subgingival plaque were determined by real-time PCR. Moreover, levels of C-reactive protein (CRP) in serum and IL-6 in gingival crevicular fluid (GCF) and serum were detected by ELISA. The correlation of blood pressure, periodontal parameters, quantities of bacteria and levels of NaNO2, CRP and IL-6 was analyzed. Results:One month after NSPT, periodontal parameters and blood pressure were decreased (P<0.05), and there were lower levels of NO in both saliva and plasma (P<0.05). In addition, NSPT decreased the amounts of total bacteria, P. gingivalis, P. intermedia, and the production of IL-6 and CRP (P<0.05). Moderate correlations were revealed between systolic blood pressure and IL-6 level in GCF, salivary NaNO2 level and CAL and there was a moderate correlation between diastolic blood pressure and total bacteria level, P. intermedia (P<0.05). Conclusions:NSPT decreased NO levels and suppressed systemic and periodontal inflammations, which provided reference for the treatment of periodontitis patients with hypertension.

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