氧化锆表面接枝绿原酸-壳聚糖复合物促成骨效果

口腔生物医学 ›› 2022, Vol. 13 ›› Issue (3): 142-147.

氧化锆表面接枝绿原酸-壳聚糖复合物促成骨效果

吴桐¹,洪高英¹,周齐悦¹,陈晨¹,谢海峰²

1. 南京医科大学附属口腔医院
2. 南京医科大学

收稿日期:2021-10-18 修回日期:2022-05-16 出版日期:2022-09-25 发布日期:2022-09-29
通讯作者: 谢海峰 E-mail:xhf-1980@126.com
基金资助:
国家自然科学基金;江苏省卫健委医学科研项目;江苏高校“青蓝工程”与优势学科建设项目

Effect of grafting chlorogenic acid-chitosan complex on zirconia implant surface on osteointegration

Received:2021-10-18 Revised:2022-05-16 Online:2022-09-25 Published:2022-09-29

摘要/Abstract

摘要： 目的：评价氧化锆表面接枝生物活性成分——绿原酸（CGA）-壳聚糖（CS）复合物对骨结合促进的效果。方法：将聚乙烯亚胺（PEI）接枝于氧化锆表面，再接枝用CS包裹后的CGA，建立生物活性涂层。采用接触角计、X射线光电子能谱、扫描电子显微镜等对涂层进行表征分析。利用具有高成骨分化潜能的小鼠前成骨细胞系MC3T3-E1评价涂层的促细胞增殖、粘附及成骨诱导作用。结果：PEI及CGA-CS复合物成功接枝于氧化锆植体表面，该涂层增强了氧化锆表面的亲水性，能促进MC3T3-E1细胞的增殖及粘附，促进MC3T3-E1表达骨钙素（Ocn）、Runt相关转录因子2（Runx2）等成骨相关蛋白。结论：氧化锆植体表面PEI涂层接枝CGA-CS复合物具有促进氧化锆表面成骨的作用。

关键词: 氧化锆植体, 绿原酸, 生物活性涂层, 成骨分化

Abstract: Objective:To evaluate the effect of zirconia surface grafted bioactive component chlorogenic acid (CGA) - chitosan (CS) complex on the promotion of osseointegration. Methods:CS wrap the CGA and graft it onto the surface of zirconia implants with PEI to build a biologically active coating. Using water contact angle measurement, X-ray photoelectron spectroscopy (XPS), scanning electron microscope (SEM), and other experiments to analyze the coating′s characterization. Pre-osteoblastic MC3T3-E1 cells with high osteogenic differentiation potential was used to evaluate the cell proliferation，adhesion promotion and osteoinduction effects of the coating. Results:PEI and CGA-CS complexes were successfully grafted on the surface of zirconia implants. The coating enhanced the hydrophilicity of zirconia surface, promoted the proliferation and adhesion of MC3T3-E1 cells, and promoted the expression of osteogenic related proteins such as Osteocalcin (Ocn) and Runt related transcription factor 2 (Runx2). Conclusions:The PEI coating grafted CGA-CS complexes can promote the osteogenesis on the surface of zirconia.

Key words: zirconia implant , chlorogenic acid , bioactive coating , osteogenic differentiation

中图分类号:

R782.12

吴桐洪高英周齐悦陈晨谢海峰. 氧化锆表面接枝绿原酸-壳聚糖复合物促成骨效果[J]. 口腔生物医学, 2022, 13(3): 142-147.

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