人腮腺区多形性腺瘤中差异表达长链非编码RNA的初步筛选分析

口腔生物医学 ›› 2024, Vol. 15 ›› Issue (6): 341-345.

人腮腺区多形性腺瘤中差异表达长链非编码RNA的初步筛选分析

许飞虎¹,张祥¹,钟志生¹,汪旭¹,丁修明¹,王爱珠¹,吴亚娟¹,杨雯君²,徐万林²

1. 海安市人民医院
2. 上海交通大学医学院附属第九人民医院

收稿日期:2024-04-17 修回日期:2024-06-23 出版日期:2024-12-25 发布日期:2025-02-25
通讯作者: 徐万林 E-mail:xwlcontribution@163.com
基金资助:
南通市科技局社会民生科技计划项目

Preliminary screen and analysis of differentially expressed lncRNAs in human parotid pleomorphic adenoma

Received:2024-04-17 Revised:2024-06-23 Online:2024-12-25 Published:2025-02-25
Contact: Wanlin Xu E-mail:xwlcontribution@163.com

摘要/Abstract

摘要： 目的：检测人腮腺区多形性腺瘤中差异表达的长链非编码RNA（lncRNA），并进行初步筛选分析。方法：收集3对人腮腺区多形性腺瘤肿瘤（肿瘤组）和瘤旁标本（对照组），运用高通量lncRNA芯片检测其差异表达的lncRNA，统计分析差异表达lncRNA的数目和分类。通过计算Pearson相关系数，筛选出与多形性腺瘤相关基因1（PLAG1）和β-连环蛋白基因1（CTNNB1）共表达的lncRNA，并利用实时荧光定量PCR对其中感兴趣lncRNA的表达进行验证。结果：相较于对照组，共计17 382个lncRNA在人腮腺区多形性腺瘤肿瘤标本中呈现显著差异（上调的8 182个，下调的9 200个），而差异表达lncRNA的主要为自然反义型和基因间型lncRNA（P＜0.05）。同时筛选出23个与PLAG1和CTNNB1共表达的lncRNA（上调的11个，下调的12个），其中lncRNA ENST00000609511在扩大样本验证中差异无统计学意义（P＞0.05），而lncRNA T256768在肿瘤标本中的表达较对照组中明显降低（P＜0.05）。结论：人腮腺区多形性腺瘤中差异表达的lncRNA T256768可与腮腺区多形性腺瘤的发生发展相关。

关键词: 多形性腺瘤, 腮腺, 唾液腺肿瘤, 长链非编码RNA, 多形性腺瘤基因1

Abstract: Objective:?To preliminarily screen and analyze the differentially expressed long non-coding RNAs in human parotid pleomorphic adenoma. Methods:?Three pairs of human parotid pleomorphic adenoma tumors (tumor group) and adjacent specimens (control group) were collected and differentially expressed lncRNAwere detected by high-throughput lncRNA-chip. By calculating the Pearson correlation coefficient (pcc), lncRNA co-expressed with pleomorphic adenoma gene 1 (PLAG1) and catenin beta 1 (CTNNB1) were screened, and the expression of interested lncRNA were verified using real-time PCR. Results: Compared to the control group, a total of 17382 lncRNA showed significant differencesin human parotid pleomorphic adenoma tumor samples; among these lncRNA, 8182 were up-regulated, and 9200 were down-regulated, which were mainly anti-sense and intergenic lncRNA (P＜0.05). Meanwhile, 23 lncRNA co-expressed with PLAG1 and CTNNB1 were screened (11 upregulated and 12 downregulated), among which ENST000000609511 showed no statistical difference in following validation (P＞0.05), while the expression of T256768 in tumor samples was significantly reduced compared to the control group (P＜0.05). Conclusions:?The differentially expression of lncRNA-T256768 may be related to the occurrence and development of parotid pleomorphic adenoma.

Key words: Pleomorphic adenoma, parotid gland, salivary gland tumor, long non-coding RNA, PLAG1

许飞虎张祥钟志生汪旭丁修明王爱珠吴亚娟杨雯君徐万林. 人腮腺区多形性腺瘤中差异表达长链非编码RNA的初步筛选分析[J]. 口腔生物医学, 2024, 15(6): 341-345.

参考文献 20

[1]	Tian Z, Li L, Wang L, et al. Salivary gland neoplasms in oral and maxillofacial regions: a 23-year retrospective study of 6982 cases in an eastern Chinese population. Int J Oral Maxillofac Surg, 2010, 39(3):235-242.
[2]	徐万林, 朱云, 卢浩, et al. 腮腺区广泛型复发性多形性腺瘤的面神经处理及术后放疗经验：附10例报告. 上海口腔医学, 2021, 30(06):667-672.
[3]	许飞虎, 徐万林, 刘胜文, et al. 腮腺区复发性多形性腺瘤恶变侵犯颧骨1例报告. 中国口腔颌面外科杂志, 2021, 19(06):573-576.
[4]	Wei C, Xu Y, Shen Q, et al. Role of long non-coding RNAs in cancer: From subcellular localization to nanoparticle-mediated targeted regulation. Molecular therapy Nucleic acids, 2023, 33:774-793.
[5]	Das PK, Siddika A, Rashel KM, et al. Roles of long noncoding RNA in triple-negative breast cancer. Cancer medicine, 2023.
[6]	Moldogazieva NT, Zavadskiy SP, Astakhov DV, et al. Differentially expressed non-coding RNAs and their regulatory networks in liver cancer. Heliyon, 2023, 9(9):e19223.
[7]	Baghdadi H, Heidari R, Zavvar M, et al. Long Non-Coding RNA Signatures in Lymphopoiesis and Lymphoid Malignancies. Non-coding RNA, 2023, 9(4).
[8]	Kalmatte A, Rekha PD, Ratnacaram CK. Emerging cell cycle related non-coding RNA biomarkers from saliva and blood for oral squamous cell carcinoma. Molecular biology reports, 2023.
[9]	Xu W, Liu L, Lu H, et al. Dysregulated long noncoding RNAs in pleomorphic adenoma tissues of pleomorphic adenoma gene 1 transgenic mice. Mol Med Rep, 2019, 19(6):4735-4742.
[10]	赵旭东, 杨雯珺, 王龙, et al. 多形性腺瘤基因1高表达转基因小鼠动物模型的建立. 中华医学遗传学杂志, 2003, (05):26-31.
[11]	Wang Y, Shang W, Lei X, et al. Opposing functions of PLAG1 in pleomorphic adenoma: a microarray analysis of PLAG1 transgenic mice. Biotechnology letters, 2013, 35(9):1377-1385.
[12]	Zhao X, Ren W, Yang W, et al. Wnt pathway is involved in pleomorphic adenomas induced by overexpression of PLAG1 in transgenic mice. Int J Cancer, 2006, 118(3):643-648.
[13]	Yan S, Wang M. HCG11 inhibits salivary adenoid cystic carcinoma by upregulating EphA2 via binding to miR-1297. Oral surgery, oral medicine, oral pathology and oral radiology, 2023, 135(2):257-267.
[14]	Chen CW, Fu M, Du ZH, et al. Long Noncoding RNA MRPL23-AS1 Promotes Adenoid Cystic Carcinoma Lung Metastasis. Cancer research, 2020, 80(11):2273-2285.
[15]	Li YR, Fu M, Song YQ, et al. Long non-coding RNA MRPL23-AS1 suppresses anoikis in salivary adenoid cystic carcinoma in vitro. Oral Dis, 2023, 29(4):1588-1601.
[16]	Lu H, Han N, Xu W, et al. Screening and bioinformatics analysis of mRNA, long non-coding RNA and circular RNA expression profiles in mucoepidermoid carcinoma of salivary gland. Biochemical and biophysical research communications, 2019, 508(1):66-71.
[17]	Chen Z, Lin S, Li JL, et al. CRTC1-MAML2 fusion-induced lncRNA LINC00473 expression maintains the growth and survival of human mucoepidermoid carcinoma cells. Oncogene, 2018, 37(14):1885-1895.
[18]	Martins C, Fonseca I, Roque L, et al. PLAG1 gene alterations in salivary gland pleomorphic adenoma and carcinoma ex-pleomorphic adenoma: a combined study using chromosome banding, in situ hybridization and immunocytochemistry. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 2005, 18(8):1048-1055.
[19]	Wang YP, Guo PT, Zhu Z, et al. Pleomorphic adenoma gene like-2 induces epithelial-mesenchymal transition via Wnt/β-catenin signaling pathway in human colorectal adenocarcinoma. Oncology reports, 2017, 37(4):1961-1970.
[20]	Voz ML, Agten NS, Van de Ven WJ, et al. PLAG1, the main translocation target in pleomorphic adenoma of the salivary glands, is a positive regulator of IGF-II. Cancer research, 2000, 60(1):106-113.