Abstract: Objective： To investigate the effect of dopamine and dopamine/Type I collagen on the adhesion behavior of MC3T3-E1 cells. Methods： To evaluate the cell adhsion, the surface dopamine-coated (DOPA) and dopamine/collagen surface-coated (DOPA+Col) were prepared respectively. The surface without coating was set as control. MC3T3-E1 cells were chosen to seed onto the three groups (DOPA, DOPA+Col, Control), respectively. CCK-8 were used to evaluate the cell proliferation. The adhesion of MC3T3-E1 cells were observed by field emission scanning electron microscope (FESEM), confocal laser scanning microscope (CLSM) and stereo microscope. Results： There was no difference in cell proliferation at 1 d, 3 d or 7 d (P > 0.05) in each group. For adhesion morphology, MC3T3-E1 extended and adhered more on the DOPA and DOPA+Col treated surfaces. Conclusions： It revealed that dopamine or dopamine + Type I collagen were favorable for cell adhesion. Both treatments have good application prospects in bone tissue engineering.