骨折愈合过程中蛋白聚糖增龄性变化

›› 2020, Vol. 11 ›› Issue (1): 19-23.

骨折愈合过程中蛋白聚糖增龄性变化

范琪琪,周梦琪,孙瑶

同济大学附属口腔医院

收稿日期:2019-11-01 修回日期:2020-03-05 出版日期:2020-03-25 发布日期:2020-04-07
通讯作者: 孙瑶 E-mail:yaosun@tongji.edu.cn
基金资助:
硬组织生物矿化

Age-related changes of proteoglycans during fracture healing

Received:2019-11-01 Revised:2020-03-05 Online:2020-03-25 Published:2020-04-07

摘要/Abstract

摘要： 目的：通过建立小鼠股骨骨折模型，探讨蛋白聚糖的增龄性变化对骨折愈合的影响。方法：取2月龄及9月龄C57BL/6J小鼠，分别建立右侧股骨骨折模型，术后7 d收样，组织学染色观察其骨折愈合情况，实时定量PCR检测Col2、Col10、Sox9、Mmp9等软骨形成相关基因及Dmp1、Vcan、Dcn、Bgn等蛋白聚糖相关基因表达情况。结果：成功构建了小鼠股骨骨折模型，组织学染色可见9月龄小鼠骨折后骨痂形成较2月龄小鼠差；实时定量PCR结果显示，骨折愈合过程中9月龄小鼠Col10、Sox9、Mmp9等软骨形成相关基因和Dmp1、Vcan、Dcn、Bgn等蛋白聚糖相关基因较2月龄小鼠低（P<0.05）。结论：在骨折愈合过程中，衰老小鼠蛋白聚糖表达较低，提示可能与骨折增龄性愈合减缓相关。

关键词: 骨折, 衰老, 蛋白聚糖, 软骨

Abstract: Objective: To investigate the role of age-related proteoglycans in fracture healing by establishing a model of femur fracture in mice. Methods: We established right femur fracture models on 2 months and 9 months C57BL/6J, they were sacrificed at 7 days post surgery. The fracture healing was observed by histological stain, the expression of chondrogenic genes and proteoglycan was detected by Real-time PCR. Results: The mouse femur fracture model was successfully constructed. Histological staining showed that the callus of 9 months mice was smaller than that of 2 months mice after fracture. Real-time PCR showed the expression of cartilage-related genes such as Col10, Sox9, Mmp9 and proteoglycan-related genes such as Dmp1, Vcan, Dcn, Bgn during fracture healing of 9 months mice were lower than that of 2 months mice (P<0.05). Conclusion: In the process of fracture healing, the expression of proteoglycans in aged mice is decreased, suggesting that proteoglycans may be related to the poor fracture healing in aged mice.

Key words: Bone fracture, Aging, Proteoglycan, Cartilage

范琪琪周梦琪孙瑶. 骨折愈合过程中蛋白聚糖增龄性变化[J]. 口腔生物医学, 2020, 11(1): 19-23.

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