载细胞微球在牙髓再生研究中的进展及展望

口腔生物医学 ›› 2021, Vol. 12 ›› Issue (4): 267-272.

载细胞微球在牙髓再生研究中的进展及展望

谢利¹,阳婷¹,张锐涛¹,张清源¹,田卫东²

1. 四川大学华西口腔医院
2. 口腔转化医学教育部工程中心（四川大学），口腔再生医学国家地方联合工程实验室（四川大学），口腔疾病研究国家重点实验室（四川大学），国家口腔疾病临床医学研究中心（四川大学），四川大学华西口腔医院创伤整形外科

收稿日期:2021-06-30 修回日期:2021-11-23 出版日期:2021-12-25 发布日期:2022-01-04
通讯作者: 田卫东 E-mail:drtwd@sina.com
基金资助:
国家重点研发计划项目;国家自然科学基金项目;四川省科技计划重点研发项目

Progress and Prospect of Cell-laden Microspheres in Endodontic Regeneration Research

Received:2021-06-30 Revised:2021-11-23 Online:2021-12-25 Published:2022-01-04
Contact: Wei-Dong TIAN E-mail:drtwd@sina.com

摘要/Abstract

摘要： 高效的细胞培养和递送载体对于基于干细胞的组织再生具有重要作用。近年来，载细胞微球作为一种新型的干细胞培养和递送支架在再生医学领域颇受关注，它们具有尺寸小、比表面积大、可微创注射、能为细胞提供3D生长环境、可一体化体外扩增和体内移植等诸多优点。由于根管系统狭窄且不规则，且血管系统的重建较为困难，具有可注射、快速物质扩散能力的载细胞微球成为牙髓再生应用的理想选择。载细胞微球可以进一步功能优化设计以解决牙髓再生中面临的难题，如增强干细胞的成牙分化，或促进血管系统在根管中的重构再生等。目前尚无中文文献就该主题进行综述总结，因此，本文拟概述载细胞微球的性质和特点，重点介绍并评述其在牙髓再生中的研究现状，最后对今后的研究方向做总结展望。

关键词: 载细胞微球, 牙髓再生, 水凝胶微囊, 体外培养, 组织工程。

Abstract: Efficient cell vehicles for cell culture and delivery play an important role in stem cell-based tissue regeneration. In recent years, as a new stem cell culture and delivery vehicles, cell-laden microspheres have attracted great attention among researchers due to their multiple advantages, including small size and large specific surface area, injectability, capacity to provide 3D growth environment for cells, integrated ability for both in vitro expanding and in vivo transplantation. For dental pulp regeneration, the root canal system is narrow and irregular, and the reconstruction of the vascular system in it is rather difficult, so the cell-laden microspheres with injectability and rapid mass diffusion became the ideal choice. What’s more, cell-laden microspheres can be optimized through material design to solve the bottleneck issues in pulp regeneration research, including enhancing the odontontic differentiation of the stem cells, or promoting the reconstruction and regeneration of vascular system in the root canal. At present, there is no Chinese review article that summarizes this topic. This paper aims to briefly introduce the properties and characteristics of the cell-laden microspheres, mainly to summarize the research progess in dental pulp regeneration fields, and make some prospects of the future research direction.

Key words: Cell-laden microspheres, endodontic regeneration, hydrogel microcapsules, in vitro culture, tissue engineering.

谢利阳婷张锐涛张清源田卫东. 载细胞微球在牙髓再生研究中的进展及展望[J]. 口腔生物医学, 2021, 12(4): 267-272.

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