Abstract:

Objective: Preparation of Nb₂C/3D printed silicon nitride (Si₃N₄) composite tissue engineering scaffolds and investigation on biological characteristics in vitro of Nb₂C MXene nanocoating. Methods: Si₃N₄ scaffold was prepared by 3D printing technology, and Nb₂C nanosheet suspension was prepared by HCl-LiF hydrothermal in situ corrosion and stripping treatment,Nb₂C biocoating was obtained by soaking the Si₃N₄ scaffold in 1.5 mg/mL Nb₂C nanosolution; Nb₂C/Si₃N₄ scaffold extracts were prepared and filtered and divided into four groups with MC3T3 cell lines, including blank control, positive control with 2% DMSO, Si₃N₄ and Nb₂C/Si₃N₄ groups, and cell proliferation activity was measured by CCK-8. Results: Compared to blank group, the proliferation activity of MC3T3 cells was significantly increased for positive control group (P＜0.05); Compared to Si₃N₄ group, the proliferation activity of MC3T3 cells was significantly increased for Nb2C/Si3N4 scaffold group (P＜0.05). Conclusions: Nb₂C/Si₃N₄ composite scaffold biolating can promote the proliferation of MC3T3 cells. It is a material with potential application for bone tissue engineering.

[Key words] Nb₂C; MXene; silicon nitride; biological coating; osteoblasts; cell proliferation; bone tissue engineering