小鼠杏仁中央核内TRPV1参与实验性正畸牙移动疼痛和焦虑的研究

口腔生物医学 ›› 2023, Vol. 14 ›› Issue (4): 224-228.

小鼠杏仁中央核内TRPV1参与实验性正畸牙移动疼痛和焦虑的研究

方丹^1,2,王芮^1,2,乔虎¹,^2*

1. 西安交通大学口腔医院陕西省颅颌面精准医学研究重点实验室,
2. 西安交通大学儿童牙颌颜面发育管理专科

收稿日期:2023-06-26 修回日期:2023-11-18 出版日期:2023-12-25 发布日期:2023-12-28
通讯作者: 乔虎 E-mail:qiaohu_xjtu@163.com
基金资助:
国家自然科学基金青年科学基金项目;西安交通大学基本科研业务费专项科研项目

Study of TRPV1 in the central nucleus of the amygdala participates in pain and anxiety of experimental orthodontic tooth movement in mice

1. 西安交通大学口腔医院
2. Stomatology Hospital of Xi’an Jiaotong University College of Medicine
3.
4. 1. Key laboratory of Shaanxi Province for Craniofacial Precision Medicine Research，College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, China; 2. Development Management in Children，Xi’an Jiaotong University, Xi’an 710004, China

Received:2023-06-26 Revised:2023-11-18 Online:2023-12-25 Published:2023-12-28

摘要/Abstract

摘要： 目的：初步探究小鼠杏仁中央核（CeA）内瞬时受体电位香草样物质1（TRPV1）是否参与牙移动疼痛及焦虑。方法：选取40只8周龄雄性C57BL/6J小鼠，按牙齿移动力值随机分为空白组、0 cN、10 cN、30 cN和50 cN组，每组8只。在不同时间点测试行为学指标，擦脸实验评估小鼠痛觉水平，旷野实验和高架十字迷宫实验评估小鼠焦虑样状态，采用免疫荧光的方法观察CeA内TRPV1的分布及表达，Western blot方法检测小鼠CeA内TRPV1蛋白表达水平在实验性牙移动过程中随时间变化趋势。结果：小鼠在实验性牙移动过程中各力值均表现出疼痛，其中30 cN组在1 d时擦脸水平最高，各力值的小鼠也表现出不同程度的焦虑样行为，30 cN组在28 d时各焦虑样指标相较于0 cN组均有差异（P<0.05）；免疫荧光染色结果显示30 cN 1 d组TRPV1的表达量高于0 cN 1 d组（P<0.001）；Western blot实验结果显示，在实验性牙移动过程中CeA内TRPV1表达在30 cN 4 h、1 d、3 d、28 d相较于0 cN 1 d组比较明显增加，且在1 d和28 d达到高峰（P<0.001）。结论：CeA内TRPV1参与实验性正畸牙移动疼痛及焦虑。

Abstract: Objective:To explore whether TRPV1 in CeA of mice was involved in tooth movement pain and anxiety. Methods:Forty 8-week-old adult male C57BL/6J mice were randomly divided into blank group, 0 cN, 10 cN, 30 cN and 50 cN groups according to the tooth movement force. Then the behavioral indexes were recorded at different time points. The pain level of mice was evaluated by face wiping test, the anxiety-like state was evaluated by open field test and elevated maze test. Immunofluorescence method was used to observe the distribution and expression of TRPV1 in CeA, western blot method was used to detect the change trend of TRPV1 protein expression in mouse CeA during orthodontic tooth movement. Results:All the force values of mice showed pain during the experimental tooth movement, in which the 30 cN group showed the highest level of face wiping on the 1st day. The mice with different force values also showed different degrees of anxiety-like behavior, and the anxiety-like indexes in the 30 cN group were significantly higher than those in the 0 cN group on the 28th day (P<0.05). Immunofluorescence staining showed that the expression of TRPV1 in the 30 cN 1 d group was higher than that in the 0 cN 1 d group (P<0.001). Western blot experiment showed that the expression of TRPV1 in CeA in 30 cN group was significantly higher than that in 0 cN 1 d group during experimental tooth movement, and reached the peak on the 1st and 28th day (P<0.05). Conclusions:TRPV1 in CeA participated in pain and anxiety of experimental tooth movement.

方丹王芮乔虎. 小鼠杏仁中央核内TRPV1参与实验性正畸牙移动疼痛和焦虑的研究[J]. 口腔生物医学, 2023, 14(4): 224-228.

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