牙源性干细胞促血管化与组织再生的研究进展

摘要/Abstract

摘要： 牙源性干细胞具有间充质干细胞特性，通过与内皮细胞相互作用或分泌多种血管生成相关细胞因子促进血管再生。目前已分离并鉴定出的牙源性干细胞主要包括牙髓干细胞、脱落乳牙干细胞、牙周膜干细胞、根尖牙乳头干细胞、牙龈间充质干细胞和牙囊干细胞。本文通过整理分析近年文献资料，对上述牙源性干细胞促血管生成与组织再生的相关研究进展进行综述。

关键词: 牙源性干细胞, 间充质干细胞, 血管生成, 组织再生

Abstract: As a kind of mesenchymal stem cells, dental stem cells can promote angiogenesis by interacting with endothelial cells or secreting a variety of angiogenesis-related cytokines. Several major types of dental stem cells have been isolated and characterized, including dental pulp stem cells, exfoliated deciduous teeth stem cells, periodontal ligament stem cells, apical papilla stem cells, gingival mesenchymal stem cells and dental follicle stem cells. Based on the analysis of the literature in recent years, this paper summarizes and reviews the related research progress of the above-mentioned dental stem cells in promoting angiogenesis and tissue regeneration.

Key words: dental stem cells, mesenchymal stem cells, angiogenesis, tissue regeneration

中图分类号:

汪杰吴偲杨德琴. 牙源性干细胞促血管化与组织再生的研究进展[J]. 口腔生物医学, 2024, 15(4): 235-239.

参考文献 48

[1]	Eelen G, Treps L, Li X, Carmeliet P. Basic and Therapeutic Aspects of Angiogenesis Updated[J]. Circ Res, 2020, 127(2)：310-329.
[2]	Liu ZL, Chen HH, Zheng LL, Sun LP, Shi L. Angiogenic signaling pathways and anti-angiogenic therapy for cancer[J]. Signal Transduct Target Ther, 2023,8(1):198.
[3]	Suchánek J, Soukup T, Ivancaková R, et al. Human dental pulp stem cells--isolation and long term cultivation[J]. Acta Medica (Hradec Kralove), 2007, 50(3)：195-201.
[4]	Yin JY, Luo XH, Feng WQ, et al. Multidifferentiation potential of dental-derived stem cells s[J]. World J Stem Cells,2021, 13(5):342-365.
[5]	Li B, Ouchi T, Cao Y, Zhao Z, Men Y. Dental-Derived Mesenchymal Stem Cells: State of the Art[J]. Front Cell Dev Biol, 2021, 9:654559.
[6]	Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo[J]. Proc Natl Acad Sci U S A, 2000, 97(25)：13625-13630.
[7]	Miura M, Gronthos S, Zhao M, et al. SHED: stem cells from human exfoliated deciduous teeth[J]. Proc Natl Acad Sci U S A, 2003,100(10)：5807-5812.
[8]	Seo BM, Miura M, Gronthos S, et al. Investigation of multipotent postnatal stem cells from human periodontal ligament[J]. Lancet, 2004, 364(9429)：149-155.
[9]	Sonoyama W, Liu Y, Fang D, et al. Mesenchymal stem cell-mediated functional tooth regeneration in swine[J]. PLoS One, 2006, 1(1)：e79.
[10]	Sonoyama W, Liu Y, Yamaza T, et al. Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study[J]. J Endod, 2008, 34(2)：166-171.
[11]	Jin S H, Lee J E, Yun J-H, et al. Isolation and characterization of human mesenchymal stem cells from gingival connective tissue[J]. J Periodontal Res, 2015, 50(4)：461-467
[12]	Morsczeck C, G?tz W, Schierholz J, et al. Isolation of precursor cells (PCs) from human dental follicle of wisdom teeth[J]. Matrix Biol, 2005, 24(2)：155-165.
[13]	Xie Z, Shen Z, Zhan P, et al. Functional Dental Pulp Regeneration: Basic Research and Clinical Translation[J]. Int J Mol Sci, 2021, 22(16)：8991.
[14]	胡佳，王秀清，卢国英，等.再生性牙髓治疗在成人根尖发育不全恒牙应用的研究进展[J].国际口腔医学杂志，2023，50(06)：686-695.
[15]	Guo H, Li B, Wu M, et al. Odontogenesis-related developmental microenvironment facilitates deciduous dental pulp stem cell aggregates to revitalize an avulsed tooth[J]. Biomaterials, 2021, 279: 121223.
[16]	Zhang Z, Oh M, Sasaki JI, N?r JE. Inverse and reciprocal regulation of p53/p21 and Bmi-1 modulates vasculogenic differentiation of dental pulp stem cells[J]. Cell Death Dis, 2021, 12(7):644.
[17]	Zhang Y, Liu J, Zou T, et al. DPSCs treated by TGF-β1 regulate angiogenic sprouting of three-dimensionally co-cultured HUVECs and DPSCs through VEGF-Ang-Tie2 signaling[J]. Stem Cell Res Ther, 2021, 12(1):281.
[18]	Guo H, Zhao W, Liu A, et al. SHED promote angiogenesis in stem cell-mediated dental pulp regeneration[J]. Biochem Biophys Res Commun, 2020, 529(4):1158-1164.
[19]	Zaw Su Yee Myo, Kaneko Tomoatsu, Zaw Zar Chi Thein, et al. Crosstalk between dental pulp stem cells and endothelial cells augments angiogenic factor expression[J]. Oral Dis, 2020, 26(6):1275-1283
[20]	Yeasmin S, Ceccarelli J, Vigen M, et al. Stem cells derived from tooth periodontal ligament enhance functional angiogenesis by endothelial cells[J]. Tissue Eng Part A, 2014, 20(7-8):1188-1196.
[21]	Zhang Z, Shuai Y, Zhou F, et al. PDLSCs Regulate Angiogenesis of Periodontal Ligaments via VEGF Transferred by Exosomes in Periodontitis[J]. Int J Med Sci, 2020, 17(5):558-567.
[22]	Zhang Z, Deng M, Hao M, Tang J. Periodontal ligament stem cells in the periodontitis niche: inseparable interactions and mechanisms[J]. J Leukoc Biol, 2021, 110(3):565-576.
[23]	Komaki M. Pericytes in the Periodontal Ligament[J]. Adv Exp Med Biol, 2019,1122:169-186.
[24]	Gong X, Zhang H, Xu X, et al. Tracing PRX1+ cells during molar formation and periodontal ligament reconstruction[J]. Int J Oral Sci, 2022, 14(1):5.
[25]	Li L, Shang L, Kang W, Du L, Ge S. Neuregulin-1 promotes the proliferation, migration, and angiogenesis of human periodontal ligament stem cells in vitro[J]. Cell Biol Int, 2022, 46(5):792-805.
[26]	Iwasaki K, Akazawa K, Nagata M, et al. Angiogenic Effects of Secreted Factors from Periodontal Ligament Stem Cells[J]. Dent J (Basel), 2021, 9(1):9.
[27]	Bakopoulou A, Kritis A, Andreadis D, et al. Angiogenic Potential and Secretome of Human Apical Papilla Mesenchymal Stem Cells in Various Stress Microenvironments[J]. Stem Cells Dev, 2015, 24(21):2496-2512.
[28]	Hilkens P, Fanton Y, Martens W, et al. Pro-angiogenic impact of dental stem cells in vitro and in vivo[J]. Stem Cell Res, 2014, 12(3):778-790.
[29]	Yuan C, Wang P, Zhu S, et al. Overexpression of ephrinB2 in stem cells from apical papilla accelerates angiogenesis[J]. Oral Dis, 2019, 25(3):848-859.
[30]	Chrepa V, Pitcher B, Henry MA, Diogenes A. Survival of the Apical Papilla and Its Resident Stem Cells in a Case of Advanced Pulpal Necrosis and Apical Periodontitis[J]. Journal of endodontics, 2017, 43(4):561-567.
[31]	Yang K, Xie D, Lin W, Xiang P, Peng C. Adipose mesenchymal stem cells and gingival mesenchymal stem cells have a comparable effect in endothelium repair[J]. Exp Ther Med,2021,22(6):1415.
[32]	Müller P, Ekat K, Brosemann A, et al. Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells[J]. J Vis Exp,2018,141：e58089.
[33]	Hade MD, Suire CN, Suo Z. Mesenchymal Stem Cell-Derived Exosomes: Applications in Regenerative Medicine[J]. Cells,2021,10(8):1959.
[34]	Zhou Z, Zheng J, Lin D, Xu R, Chen Y, Hu X. Exosomes derived from dental pulp stem cells accelerate cutaneous wound healing by enhancing angiogenesis via the Cdc42/p38 MAPK pathway[J]. Int J Mol Med, 2022,50(6):143.
[35]	Huang X, Qiu W, Pan Y, et al. Exosomes from LPS-Stimulated hDPSCs Activated the Angiogenic Potential of HUVECs In Vitro[J]. Stem Cells Int, 2021,2021:6685307.
[36]	Chen WJ, Xie J, Lin X, et al. The Role of Small Extracellular Vesicles Derived from Lipopolysaccharide-preconditioned Human Dental Pulp Stem Cells in Dental Pulp Regeneration[J]. J Endod, 2021,47(6):961-969.
[37]	Zhou H, Li X, Wu RX, et al. Periodontitis-compromised dental pulp stem cells secrete extracellular vesicles carrying miRNA-378a promote local angiogenesis by targeting Sufu to activate the Hedgehog/Gli1 signalling[J]. Cell Prolif, 2021,54(5):e13026.
[38]	Wu M, Liu X, Li Z, et al. SHED aggregate exosomes shuttled miR-26a promote angiogenesis in pulp regeneration via TGF-β/SMAD2/3 signalling[J]. Cell Prolif, 2021, 54(7):e13074.
[39]	Gao Y, Yuan Z, Yuan X, et al. Bioinspired porous microspheres for sustained hypoxic exosomes release and vascularized bone regeneration[J]. Bioact Mater, 2022, 14: 377-388.
[40]	Liu Y, Zhuang X, Yu S, et al. Exosomes derived from stem cells from apical papilla promote craniofacial soft tissue regeneration by enhancing Cdc42-mediated vascularization[J]. Stem Cell Res Ther, 2021, 12(1):76.
[41]	Jing X, Wang S, Tang H, et al. Dynamically Bioresponsive DNA Hydrogel Incorporated with Dual-Functional Stem Cells from Apical Papilla-Derived Exosomes Promotes Diabetic Bone Regeneration[J]. ACS Appl Mater Interfaces, 2022,14(14):16082-16099.
[42]	Greene CJ, Anderson S, Barthels D, et al. DPSC Products Accelerate Wound Healing in Diabetic Mice through Induction of SMAD Molecules[J]. Cells,2022,11(15):2409.
[43]	Wang F, Li Z, Lyu FJ, et al. The therapeutic effect of stem cells from human exfoliated deciduous teeth on a rat model of tracheal fistula [published correction appears in Stem Cell Res Ther[J]. Stem Cell Res Ther,2022,13(1):310.
[44]	Jing X, Wang S, Tang H, et al. Dynamically Bioresponsive DNA Hydrogel Incorporated with Dual-Functional Stem Cells from Apical Papilla-Derived Exosomes Promotes Diabetic Bone Regeneration[J]. ACS Appl Mater Interfaces, 2022, 14(14):16082-16099.
[45]	Bergamo M T, Zhang Z, Oliveira T M et al. VEGFR1 primes a unique cohort of dental pulp stem cells for vasculogenic differentiation[J]. Eur Cell Mater, 2021, 41: 332-344.
[46]	Ding T, Kang W, Li J, Yu L, Ge S. An in situ tissue engineering scaffold with growth factors combining angiogenesis and osteoimmunomodulatory functions for advanced periodontal bone regeneration[J]. J Nanobiotechnology,2021,19(1):247.
[47]	Zhao Z, Sun Y, Qiao Q, et al. Human Periodontal Ligament Stem Cell and Umbilical Vein Endothelial Cell Co-Culture to Prevascularize Scaffolds for Angiogenic and Osteogenic Tissue Engineering[J].Int J Mol Sci, 2021,22(22):12363.
[48]	Liu J, Zou T, Yao Q, Zhang Y, Zhao Y, Zhang C. Hypoxia-mimicking cobalt-doped multi-walled carbon nanotube nanocomposites enhance the angiogenic capacity of stem cells from apical papilla[J]. Mater Sci Eng C Mater Biol Appl,2021,120:111797.