›› 2012, Vol. 3 ›› Issue (3): 120-123.

Previous Articles     Next Articles

The effect of FOXM1 siRNA on proliferation of salivary adenoid cystic carcinoma cells

  

  • Received:2012-08-03 Revised:2012-08-16 Online:2012-09-25 Published:2012-10-08

Abstract: Objective:To study the effect of FOXM1 gene on proliferation of salivary adenoid cystic carcinoma cells. Methods:The highly metastatic adenoid cystic carcinoma cell line (SACC-M) was transient transfected by constructing FOXM1 siRNA interference clips. The interference efficiency was examined by Western blotting. Cell cycle changes were determined with flow cytometry. Cell proliferation was evaluated by MTT. The protein expression of CyclinB, CDC2 was examined by Western blotting. Cell migration was examined by wound-healing assay. Results:FOXM1 siRNA transfection specifically down-regulated the FOXM1 protein in SACC-M cells. Proliferation of SACC-M cells was suppressed,and the percentage of S stage cells was significantly reduced. Cells migration did not show a clear change. Conclusions:FOXM1 could regulate cell proliferation of salivary adenoid cystic carcinoma cells, but had no obvious effects on cell migration.

CLC Number: