›› 2012, Vol. 3 ›› Issue (4): 185-188.

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Effect of 17β-estrodial on the expression of RANKL and OPG in human stem cells from apical papillae

  

  • Received:2012-10-24 Revised:2012-11-21 Online:2012-12-25 Published:2013-01-10

Abstract: [Abstract] Objective:To evaluate the effects of 17β-estrodial on the expression of receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) in human stem cells from apical papillae (SCAPs). Methods:SCAPs cultured in L-MEM containing 10 μmol/L alcohol, L-MEM containing 0.1 μmol/L 17β-estrodial for 3 days and 7 days were respectively collected to evaluate the expression of RANKL and OPG at the gene or protein level by real-time RT-PCR and Western blotting. Results:17β-estrodial can enhance the expression of OPG and downregulate the expression of RANKL. Conclusions:17β-estrodial may regulate the odonto/osteogenic and odonto/osteoclastic potential through RANKL/OPG system.