›› 2013, Vol. 4 ›› Issue (2): 65-69.

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Study on the proliferation and osteo/odongenic differentiation of human periodontal ligament stem cells in different developing stages

  

  • Received:2013-04-02 Revised:2013-05-28 Online:2013-06-25 Published:2013-06-26

Abstract: Objective:To investigate the proliferation and osteo/odongenic differentiation of human periodontal ligament stem cells (PDLSC) in different developing stages, and to provide the candidate cell sources for tooth engineering. Methods:Cells were respectively isolated from developing teeth with open apical foramen and developed teeth with closed apical foramen. Methyltetrazolium (MTT) assay was performed to observe the proliferation features of the two kinds of human periodontal ligament stem cells. And their alkaline phosphotase (ALP) activities were evaluated using an ALP assay kit. Alizarin red staining was used to observe the mineralization abilities of the two kinds of cells. Furthermore, dentin matrix protein 1 (DMP1), dentin sialoprotein (DSP), runt-related transcription factor 2 (RUNX2) and osterix (OSX) were assessed by Western blot to evaluate the osteo/odongenic differentiation capacities of two kinds of PDLSC. Results: PDLSC from developing teeth presented with a faster proliferation rate, higher ALP activity and mineralization ability, and upregulated proteins expression of DMP1, RUNX2, DSP and OSX in vitro, as compared with PDLSC from developed teeth(P <0.05). Conclusions:The developing stages can affect the proliferation and osteo/odongenic differentiation of PDLSC. After the completion of root development, the proliferation ability and osteo/odongenic differentiation capacity of PDLSC decreased due to the changes in the microenvironment at different developing stages.