组蛋白去乙酰化酶8对大鼠骨髓间充质干细胞成骨分化的影响

Abstract

Abstract: Objective：To evaluate the effect of histone deacetylase 8 (HDAC8) on osteoblast differentiation of rat bone marrow mesenchymal stem cells (BMSCs). Methods：Rat BMSCs were isolated and cultured by the method of whole bone marrow adherent and transfected with HDAC8 overexpression lentiviral vector. Real-time PCR, Western blot were applied to estimate the change of osteogenic capacity after 7 days of osteogenic induction and Alizarin red stain was used to estimate the mineralization capacity after 14 days of osteogenic induction. Trichostatin A (TSA), one kind of histone deacetylase inhibitor, acted on BMSCs with HDAC8 overexpression and the change of osteogenic capacity of cells after 7 days of osteogenic induction was estimated by real-time PCR and Western blot. Results：For BMSCs with HDAC8 overexpression, the expression of osteogenesis-related genes at mRNA and protein levels were all lower than those in control group after 7 days of ostogenic induction and the capacity to form calcified nodules was lower than that of the control group after 14 days of ostogenic induction. The osteogenesis-related genes expression of BMSCs with HDAC8 overexpression which was treated with TSA were all higher than those in untreated group after 7 days of ostogenic induction (P<0.05). Conclusions：HDAC8 could suppress osteogenic differentiation of rat BMSCs.

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References

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Effect of histone deacetylase 8 on osteoblast differentiation of rat bone marrow mesenchymal stem cells

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